Characterization of tributyrin hydrolysis by immobilized lipase on woolen cloth using conventional batch and novel spinning cloth disc reactors

X. Feng, D.A. Patterson, M. Balaban, E A C Emanuelsson

Research output: Contribution to journalArticle

18 Citations (Scopus)
106 Downloads (Pure)

Abstract

Optimal loading and operating conditions for a new, superior immobilization of amano lipase from Pseudomonas fluorescens on woolen cloth were determined. The optimal enzyme loading was 46.8 mg g dry cloth with activity of 200 U. A batch reactor was used to characterize process conditions important to industrial application of the wool immobilized lipase. The optimal pH for immobilized lipase in tributyrin hydrolysis was 7, slightly lower than that of free lipase (pH 8). The optimal temperature for both free and immobilized lipase was 45 °C. The immobilized lipase was more stable to reuse than some other lipase immobilizations, maintaining 85% of its activity after 6 long term runs and 75.8% of the original activity after storage of 40 weeks at 4 °C. The thermal stability of lipase was improved by 2.4 times after immobilization. The thermal deactivation rate of immobilized lipase followed the Arrhenius law with E = 199 kJ mol. The Michaelis-Menten constant (K ) of the lipase increased from 1.63 mM to 4.48 mM after immobilization. The immobilized lipase was also successfully applied for tributyrin hydrolysis in a novel enzyme process intensification technology - the spinning cloth disc reactor (SCDR): conversion increased by around 13% under similar conditions compared to a conventional batch stirred tank reactor. The SCDR is therefore key to exploiting the advantages of the wool immobilized lipase developed in this work.
Original languageEnglish
Pages (from-to)1684-1692
Number of pages9
JournalChemical Engineering Research & Design
Volume91
Issue number9
Early online date14 Jun 2013
DOIs
Publication statusPublished - Sep 2013

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Spinning (fibers)
Lipases
Lipase
Hydrolysis
Wool
tributyrin
Enzymes
Batch reactors
Industrial applications
Thermodynamic stability

Cite this

Characterization of tributyrin hydrolysis by immobilized lipase on woolen cloth using conventional batch and novel spinning cloth disc reactors. / Feng, X.; Patterson, D.A.; Balaban, M.; Emanuelsson, E A C.

In: Chemical Engineering Research & Design, Vol. 91, No. 9, 09.2013, p. 1684-1692.

Research output: Contribution to journalArticle

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