Characterization of Salmonella enterica serovar Typhimurium from marine environments in coastal waters of Galicia (Spain)

J. Martinez-Urtaza, E. Liebana, L. Garcia-Migura, P. Perez-Piñeiro, M. Saco

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Twenty-three Salmonella enterica serovar Typhimurium isolates from marine environments were characterized by phage typing, pulsed-field gel electrophoresis (PFGE) analysis, plasmid analysis, and antibiotic resistance, and the distribution of the different types in the coastal waters were subsequently analyzed. Five phage types were identified among the isolates (PT41, PT135, PT99, DT104, and DT193). PT135 isolates were exclusively detected during the winter months from 1998 to 2000, whereas DT104 and PT41 isolates were detected exclusively in the summer months from 2000 to 2002. XbaI PFGE analysis revealed 9 PFGE types, and plasmid profiling identified 8 plasmid types (with 1 to 6 plasmids) among the isolates. Only three isolates presented multidrug resistance to antibiotics. Two DT104 isolates were resistant to 8 and 7 antibiotics (profiles ACCeFNaSSuT and ACeFNeSSuT), whereas a PT193 isolate presented resistance to 6 antibiotics (profile ACFSSu). In addition, four PT41 isolates were resistant to a single antibiotic. The detection of multidrug-resistant phage types DT104 and DT193 in shellfish emphasizes the importance of monitoring the presence of Salmonella in routine surveillance of live bivalve molluscs.
Original languageEnglish
Pages (from-to)4030-4034
Number of pages5
JournalApplied and Environmental Microbiology
Volume70
Issue number7
DOIs
Publication statusPublished - 1 Jul 2004

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Salmonella enterica
plasmid
Salmonella Typhimurium
marine environment
antibiotics
Spain
coastal water
Pulsed Field Gel Electrophoresis
plasmids
Plasmids
pulsed-field gel electrophoresis
bacteriophages
Anti-Bacterial Agents
electrokinesis
gel
Water
Bacteriophages
Bacteriophage Typing
Shellfish
antibiotic resistance

Cite this

Characterization of Salmonella enterica serovar Typhimurium from marine environments in coastal waters of Galicia (Spain). / Martinez-Urtaza, J.; Liebana, E.; Garcia-Migura, L.; Perez-Piñeiro, P.; Saco, M.

In: Applied and Environmental Microbiology, Vol. 70, No. 7, 01.07.2004, p. 4030-4034.

Research output: Contribution to journalArticle

Martinez-Urtaza, J. ; Liebana, E. ; Garcia-Migura, L. ; Perez-Piñeiro, P. ; Saco, M. / Characterization of Salmonella enterica serovar Typhimurium from marine environments in coastal waters of Galicia (Spain). In: Applied and Environmental Microbiology. 2004 ; Vol. 70, No. 7. pp. 4030-4034.
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