Characterization of Folding Cores in the Cyclophilin A-Cyclosporin A Complex

Jack W. Heal, Stephen A. Wells, Claudia A. Blindauer, Robert B. Freedman, Rudolf A. Roemer

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Determining the folding core of a protein yields information about its folding process and dynamics. The experimental procedures for identifying the amino acids that make up the folding core include hydrogen-deuterium exchange and F-value analysis and can be expensive and time consuming. Because of this, there is a desire to improve upon existing methods for determining protein folding cores theoretically. We have obtained HDX data for the complex of cyclophilin A with the immunosuppressant cyclosporin A. We compare these data, as well as literature values for uncomplexed cyclophilin A, to theoretical predictions using a combination of rigidity analysis and coarse-grained simulations of protein motion. We find that in this case, the most specific prediction of folding cores comes from a combined approach that models the rigidity of the protein using the First software suite and the dynamics of the protein using the FRODA tool.
Original languageEnglish
Pages (from-to)1739-1746
JournalBiophysical Journal
Issue number7
Publication statusPublished - 7 Apr 2015


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