Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum

Louise F Thatcher, Volkan Cevik, Murray Grant, Bing Zhai, Jonathan D G Jones, John M Manners, Kemal Kazan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In Arabidopsis, jasmonate (JA)-signaling plays a key role in mediating Fusarium oxysporum disease outcome. However, the roles of JASMONATE ZIM-domain (JAZ) proteins that repress JA-signaling have not been characterized in host resistance or susceptibility to this pathogen. Here, we found most JAZ genes are induced following F. oxysporum challenge, and screening T-DNA insertion lines in Arabidopsis JAZ family members identified a highly disease-susceptible JAZ7 mutant (jaz7-1D). This mutant exhibited constitutive JAZ7 expression and conferred increased JA-sensitivity, suggesting activation of JA-signaling. Unlike jaz7 loss-of-function alleles, jaz7-1D also had enhanced JA-responsive gene expression, altered development and increased susceptibility to the bacterial pathogen PstDC3000 that also disrupts host JA-responses. We also demonstrate that JAZ7 interacts with transcription factors functioning as activators (MYC3, MYC4) or repressors (JAM1) of JA-signaling and contains a functional EAR repressor motif mediating transcriptional repression via the co-repressor TOPLESS (TPL). We propose through direct TPL recruitment, in wild-type plants JAZ7 functions as a repressor within the JA-response network and that in jaz7-1D plants, misregulated ectopic JAZ7 expression hyper-activates JA-signaling in part by disturbing finely-tuned COI1-JAZ-TPL-TF complexes.

Original languageEnglish
Pages (from-to)2367-2386
Number of pages20
JournalJournal of Experimental Botany
Volume67
Issue number8
DOIs
Publication statusPublished - Apr 2016

Fingerprint

Fusarium
Fusarium oxysporum
Arabidopsis
mutants
pathogens
transcription factors
screening
alleles
gene expression
DNA
genes
proteins
jasmonic acid
Co-Repressor Proteins
Transcription Factors
Alleles
Gene Expression

Keywords

  • Amino Acid Motifs
  • Arabidopsis
  • Arabidopsis Proteins
  • Co-Repressor Proteins
  • Cyclopentanes
  • DNA, Bacterial
  • Disease Resistance
  • Disease Susceptibility
  • Flowers
  • Fusarium
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Models, Biological
  • Mutagenesis, Insertional
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Oxylipins
  • Phenotype
  • Plant Diseases
  • Plants, Genetically Modified
  • Protein Binding
  • Pseudomonas syringae
  • RNA, Messenger
  • Repressor Proteins
  • Up-Regulation
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum. / Thatcher, Louise F; Cevik, Volkan; Grant, Murray; Zhai, Bing; Jones, Jonathan D G; Manners, John M; Kazan, Kemal.

In: Journal of Experimental Botany, Vol. 67, No. 8, 04.2016, p. 2367-2386.

Research output: Contribution to journalArticle

Thatcher, Louise F ; Cevik, Volkan ; Grant, Murray ; Zhai, Bing ; Jones, Jonathan D G ; Manners, John M ; Kazan, Kemal. / Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum. In: Journal of Experimental Botany. 2016 ; Vol. 67, No. 8. pp. 2367-2386.
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abstract = "In Arabidopsis, jasmonate (JA)-signaling plays a key role in mediating Fusarium oxysporum disease outcome. However, the roles of JASMONATE ZIM-domain (JAZ) proteins that repress JA-signaling have not been characterized in host resistance or susceptibility to this pathogen. Here, we found most JAZ genes are induced following F. oxysporum challenge, and screening T-DNA insertion lines in Arabidopsis JAZ family members identified a highly disease-susceptible JAZ7 mutant (jaz7-1D). This mutant exhibited constitutive JAZ7 expression and conferred increased JA-sensitivity, suggesting activation of JA-signaling. Unlike jaz7 loss-of-function alleles, jaz7-1D also had enhanced JA-responsive gene expression, altered development and increased susceptibility to the bacterial pathogen PstDC3000 that also disrupts host JA-responses. We also demonstrate that JAZ7 interacts with transcription factors functioning as activators (MYC3, MYC4) or repressors (JAM1) of JA-signaling and contains a functional EAR repressor motif mediating transcriptional repression via the co-repressor TOPLESS (TPL). We propose through direct TPL recruitment, in wild-type plants JAZ7 functions as a repressor within the JA-response network and that in jaz7-1D plants, misregulated ectopic JAZ7 expression hyper-activates JA-signaling in part by disturbing finely-tuned COI1-JAZ-TPL-TF complexes.",
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T1 - Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum

AU - Thatcher, Louise F

AU - Cevik, Volkan

AU - Grant, Murray

AU - Zhai, Bing

AU - Jones, Jonathan D G

AU - Manners, John M

AU - Kazan, Kemal

N1 - © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

PY - 2016/4

Y1 - 2016/4

N2 - In Arabidopsis, jasmonate (JA)-signaling plays a key role in mediating Fusarium oxysporum disease outcome. However, the roles of JASMONATE ZIM-domain (JAZ) proteins that repress JA-signaling have not been characterized in host resistance or susceptibility to this pathogen. Here, we found most JAZ genes are induced following F. oxysporum challenge, and screening T-DNA insertion lines in Arabidopsis JAZ family members identified a highly disease-susceptible JAZ7 mutant (jaz7-1D). This mutant exhibited constitutive JAZ7 expression and conferred increased JA-sensitivity, suggesting activation of JA-signaling. Unlike jaz7 loss-of-function alleles, jaz7-1D also had enhanced JA-responsive gene expression, altered development and increased susceptibility to the bacterial pathogen PstDC3000 that also disrupts host JA-responses. We also demonstrate that JAZ7 interacts with transcription factors functioning as activators (MYC3, MYC4) or repressors (JAM1) of JA-signaling and contains a functional EAR repressor motif mediating transcriptional repression via the co-repressor TOPLESS (TPL). We propose through direct TPL recruitment, in wild-type plants JAZ7 functions as a repressor within the JA-response network and that in jaz7-1D plants, misregulated ectopic JAZ7 expression hyper-activates JA-signaling in part by disturbing finely-tuned COI1-JAZ-TPL-TF complexes.

AB - In Arabidopsis, jasmonate (JA)-signaling plays a key role in mediating Fusarium oxysporum disease outcome. However, the roles of JASMONATE ZIM-domain (JAZ) proteins that repress JA-signaling have not been characterized in host resistance or susceptibility to this pathogen. Here, we found most JAZ genes are induced following F. oxysporum challenge, and screening T-DNA insertion lines in Arabidopsis JAZ family members identified a highly disease-susceptible JAZ7 mutant (jaz7-1D). This mutant exhibited constitutive JAZ7 expression and conferred increased JA-sensitivity, suggesting activation of JA-signaling. Unlike jaz7 loss-of-function alleles, jaz7-1D also had enhanced JA-responsive gene expression, altered development and increased susceptibility to the bacterial pathogen PstDC3000 that also disrupts host JA-responses. We also demonstrate that JAZ7 interacts with transcription factors functioning as activators (MYC3, MYC4) or repressors (JAM1) of JA-signaling and contains a functional EAR repressor motif mediating transcriptional repression via the co-repressor TOPLESS (TPL). We propose through direct TPL recruitment, in wild-type plants JAZ7 functions as a repressor within the JA-response network and that in jaz7-1D plants, misregulated ectopic JAZ7 expression hyper-activates JA-signaling in part by disturbing finely-tuned COI1-JAZ-TPL-TF complexes.

KW - Amino Acid Motifs

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Co-Repressor Proteins

KW - Cyclopentanes

KW - DNA, Bacterial

KW - Disease Resistance

KW - Disease Susceptibility

KW - Flowers

KW - Fusarium

KW - Gene Expression Regulation, Plant

KW - Genes, Plant

KW - Models, Biological

KW - Mutagenesis, Insertional

KW - Mutation

KW - Oligonucleotide Array Sequence Analysis

KW - Oxylipins

KW - Phenotype

KW - Plant Diseases

KW - Plants, Genetically Modified

KW - Protein Binding

KW - Pseudomonas syringae

KW - RNA, Messenger

KW - Repressor Proteins

KW - Up-Regulation

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

UR - https://doi.org/10.1186/s12915-016-0228-7

U2 - 10.1093/jxb/erw040

DO - 10.1093/jxb/erw040

M3 - Article

VL - 67

SP - 2367

EP - 2386

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 8

ER -