Characterization of [3H]Harmane Binding to Rat Whole Brain Membranes

N J Anderson; E S J Robinson; Stephen M Husbands; P Delagrange; D J Nutt; A L Hudson

doi:10.1196/annals.1304.020

Characterization of [3H]Harmane Binding to Rat Whole Brain Membranes

N J Anderson, E S J Robinson, Stephen M Husbands, P Delagrange, D J Nutt, A L Hudson

Department of Life Sciences

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10 Citations (SciVal)

Abstract

This study investigates the binding of [3H]harmane to rat whole brain homogenates. Saturation studies revealed [3H]harmane labels a single, saturable, high-capacity population with high affinity. All the test compounds displaced [3H]harmane completely and in an apparently monophasic manner. The displacement profile of the test ligands indicated labeling of MAO-A. Given the high level of MAO-A binding, it is unlikely that a low-capacity I2 site would be distinguishable from the total [3H]harmane population.

Original language	English
Pages (from-to)	175-179
Number of pages	5
Journal	Annals of the New York Academy of Sciences
Volume	1009
Issue number	1
DOIs	https://doi.org/10.1196/annals.1304.020
Publication status	Published - 2003

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10.1196/annals.1304.020

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title = "Characterization of [3H]Harmane Binding to Rat Whole Brain Membranes",

abstract = "This study investigates the binding of [3H]harmane to rat whole brain homogenates. Saturation studies revealed [3H]harmane labels a single, saturable, high-capacity population with high affinity. All the test compounds displaced [3H]harmane completely and in an apparently monophasic manner. The displacement profile of the test ligands indicated labeling of MAO-A. Given the high level of MAO-A binding, it is unlikely that a low-capacity I2 site would be distinguishable from the total [3H]harmane population.",

author = "Anderson, {N J} and Robinson, {E S J} and Husbands, {Stephen M} and P Delagrange and Nutt, {D J} and Hudson, {A L}",

year = "2003",

doi = "10.1196/annals.1304.020",

language = "English",

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pages = "175--179",

journal = "Annals of the New York Academy of Sciences",

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T1 - Characterization of [3H]Harmane Binding to Rat Whole Brain Membranes

AU - Anderson, N J

AU - Robinson, E S J

AU - Husbands, Stephen M

AU - Delagrange, P

AU - Nutt, D J

AU - Hudson, A L

PY - 2003

Y1 - 2003

N2 - This study investigates the binding of [3H]harmane to rat whole brain homogenates. Saturation studies revealed [3H]harmane labels a single, saturable, high-capacity population with high affinity. All the test compounds displaced [3H]harmane completely and in an apparently monophasic manner. The displacement profile of the test ligands indicated labeling of MAO-A. Given the high level of MAO-A binding, it is unlikely that a low-capacity I2 site would be distinguishable from the total [3H]harmane population.

AB - This study investigates the binding of [3H]harmane to rat whole brain homogenates. Saturation studies revealed [3H]harmane labels a single, saturable, high-capacity population with high affinity. All the test compounds displaced [3H]harmane completely and in an apparently monophasic manner. The displacement profile of the test ligands indicated labeling of MAO-A. Given the high level of MAO-A binding, it is unlikely that a low-capacity I2 site would be distinguishable from the total [3H]harmane population.

UR - http://dx.doi.org/10.1196/annals.1304.020

U2 - 10.1196/annals.1304.020

DO - 10.1196/annals.1304.020

M3 - Article

SN - 0077-8923

VL - 1009

SP - 175

EP - 179

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

IS - 1

ER -

Characterization of [3H]Harmane Binding to Rat Whole Brain Membranes

Abstract

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