Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

C Kupper; G J Horsburgh; D A Dawson; R Ffrench-Constant; T Szekely; T Burke

doi:10.1111/j.1471-8286.2006.01517.x

Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

C Kupper, G J Horsburgh, D A Dawson, R Ffrench-Constant, T Szekely, T Burke

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

42 Citations (SciVal)

Abstract

We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).

Original language	English
Pages (from-to)	35-39
Number of pages	5
Journal	Molecular Ecology Notes
Volume	7
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01517.x
Publication status	Published - 2007

Bibliographical note

ID number: ISI:000243024000010

Access to Document

10.1111/j.1471-8286.2006.01517.x

Cite this

Kupper, C., Horsburgh, G. J., Dawson, D. A., Ffrench-Constant, R., Szekely, T., & Burke, T. (2007). Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species. Molecular Ecology Notes, 7(1), 35-39. https://doi.org/10.1111/j.1471-8286.2006.01517.x

Kupper, C, Horsburgh, GJ, Dawson, DA, Ffrench-Constant, R, Szekely, T & Burke, T 2007, 'Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species', Molecular Ecology Notes, vol. 7, no. 1, pp. 35-39. https://doi.org/10.1111/j.1471-8286.2006.01517.x

@article{a9cc3b74b7684e9da4da80514d0dfc3f,

title = "Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species",

abstract = "We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).",

author = "C Kupper and Horsburgh, \{G J\} and Dawson, \{D A\} and R Ffrench-Constant and T Szekely and T Burke",

note = "ID number: ISI:000243024000010",

year = "2007",

doi = "10.1111/j.1471-8286.2006.01517.x",

language = "English",

volume = "7",

pages = "35--39",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

}

TY - JOUR

T1 - Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

AU - Kupper, C

AU - Horsburgh, G J

AU - Dawson, D A

AU - Ffrench-Constant, R

AU - Szekely, T

AU - Burke, T

N1 - ID number: ISI:000243024000010

PY - 2007

Y1 - 2007

N2 - We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).

AB - We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).

U2 - 10.1111/j.1471-8286.2006.01517.x

DO - 10.1111/j.1471-8286.2006.01517.x

M3 - Article

SN - 1471-8278

VL - 7

SP - 35

EP - 39

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 1

ER -

Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

Abstract

Bibliographical note

Access to Document

Fingerprint

Cite this