Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

C Kupper; G J Horsburgh; D A Dawson; R Ffrench-Constant; T Szekely; T Burke

doi:10.1111/j.1471-8286.2006.01517.x

Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

C Kupper, G J Horsburgh, D A Dawson, R Ffrench-Constant, T Szekely, T Burke

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

42 Citations (SciVal)

Abstract

We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).

Original language	English
Pages (from-to)	35-39
Number of pages	5
Journal	Molecular Ecology Notes
Volume	7
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01517.x
Publication status	Published - 2007

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ID number: ISI:000243024000010

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Kupper, C., Horsburgh, G. J., Dawson, D. A., Ffrench-Constant, R., Szekely, T., & Burke, T. (2007). Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species. Molecular Ecology Notes, 7(1), 35-39. https://doi.org/10.1111/j.1471-8286.2006.01517.x

Kupper, C, Horsburgh, GJ, Dawson, DA, Ffrench-Constant, R, Szekely, T & Burke, T 2007, 'Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species', Molecular Ecology Notes, vol. 7, no. 1, pp. 35-39. https://doi.org/10.1111/j.1471-8286.2006.01517.x

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abstract = "We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).",

author = "C Kupper and Horsburgh, {G J} and Dawson, {D A} and R Ffrench-Constant and T Szekely and T Burke",

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AU - Horsburgh, G J

AU - Dawson, D A

AU - Ffrench-Constant, R

AU - Szekely, T

AU - Burke, T

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AB - We isolated 45 new Kentish plover (Charadrius alexandrinus) microsatellite loci. These were tested for polymorphism in 42 Kentish plovers breeding in the Cukurova Delta, Turkey. Thirty-six of the 45 loci were polymorphic with observed heterozygosity varying between 0.22 and 0.93. Genotypes of individuals of known sex indicated that two loci were sex-linked (Calex-26 is located on the Z chromosome and Calex-31 on the W chromosome). Additionally, we tested all loci for amplification in four other species of Charadridae (Kittlitz's plover, Madagascar plover, three-banded plover and white-fronted plover). On average 34 loci amplified per species (range 29-36).

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DO - 10.1111/j.1471-8286.2006.01517.x

M3 - Article

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EP - 39

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 1

ER -

Characterization of 36 polymorphic microsatellite loci in the Kentish plover (Charadrius alexandrinus) including two sex-linked loci and their amplification in four other Charadrius species

Abstract

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