Characterisation of Salmonella enterica clones carrying mcr-1 plasmids in meat products and patients in Northern Thailand using long read sequencing

Prapas Patchanee, Nipa Chokesajjawatee, Pannita Santiyanont, Phongsakorn Chuammitri, Manu Deeudom, William Monteith, Samuel K. Sheppard, Ben Pascoe, Teerarat Prasertsee

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Abstract

Salmonella spp. is an important foodborne pathogen associated with consumption of contaminated food, especially food of livestock origin. Antimicrobial resistance (AMR) in Salmonella has been reported globally and increasing AMR in food production is a major public health issue worldwide. The objective of this study was to describe the genetic relatedness among Salmonella enterica isolates, which displayed identical DNA fingerprint profiles. Ten S. enterica isolates were selected from meat and human cases with an identical rep-PCR profile of serovars Rissen (n=4), Weltevreden (n=4), and Stanley (n=2). We used long-read whole genome sequencing (WGS) on the MinION sequencing platform to type isolates and investigate in silico the presence of specific AMR genes. Antimicrobial susceptibility testing was tested by disk diffusion and gradient diffusion method to corroborate the AMR phenotype. Multidrug resistance and resistance to more than one antimicrobial agent were observed in eight and nine isolates, respectively. Resistance to colistin with an accompanying mcr-1 gene was observed among the Salmonella isolates. The analysis of core genome and whole genome MLST revealed that the Salmonella from meat and human salmonellosis were genetically related. Hence, it could be concluded that meat is one of the important sources for Salmonella infection in human.
Original languageEnglish
Article number109314
JournalInternational Journal of Food Microbiology
Volume358
Early online date21 Jun 2021
DOIs
Publication statusPublished - 16 Nov 2021

Bibliographical note

Funding Information:
This research was financially supported by the Royal Golden Jubilee (RGJ) Ph.D. Programme ( PHD/0240/2558 ) and partially supported by Chiang Mai University . The authors would like to thank all scientists at Food Biotechnology Laboratory, National Science and Technology Development Agency (NSTDA) for their contribution. We would like to express many thanks to Dr.Supapon Cheevadhanarak, Dr.Sawannee Sutheeworapong, Dr.Songsak Wattanachaisereekul, and all staffs at Systems Biology and Bioinformatics research group (SBI) at King Mongkut's University of Technology Thonburi (KMUTT) for excellent support and providing high performance computing servers.

Keywords

  • Antimicrobial resistance
  • Long-read sequencing
  • Salmonella enterica
  • Salmonellosis
  • Typing
  • Whole genome sequencing

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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