TY - JOUR
T1 - Characterisation of Salmonella enterica clones carrying mcr-1 plasmids in meat products and patients in Northern Thailand using long read sequencing
AU - Patchanee, Prapas
AU - Chokesajjawatee, Nipa
AU - Santiyanont, Pannita
AU - Chuammitri, Phongsakorn
AU - Deeudom, Manu
AU - Monteith, William
AU - Sheppard, Samuel K.
AU - Pascoe, Ben
AU - Prasertsee, Teerarat
N1 - Funding Information:
This research was financially supported by the Royal Golden Jubilee (RGJ) Ph.D. Programme ( PHD/0240/2558 ) and partially supported by Chiang Mai University . The authors would like to thank all scientists at Food Biotechnology Laboratory, National Science and Technology Development Agency (NSTDA) for their contribution. We would like to express many thanks to Dr.Supapon Cheevadhanarak, Dr.Sawannee Sutheeworapong, Dr.Songsak Wattanachaisereekul, and all staffs at Systems Biology and Bioinformatics research group (SBI) at King Mongkut's University of Technology Thonburi (KMUTT) for excellent support and providing high performance computing servers.
PY - 2021/11/16
Y1 - 2021/11/16
N2 - Salmonella spp. is an important foodborne pathogen associated with consumption of contaminated food, especially food of livestock origin. Antimicrobial resistance (AMR) in Salmonella has been reported globally and increasing AMR in food production is a major public health issue worldwide. The objective of this study was to describe the genetic relatedness among Salmonella enterica isolates, which displayed identical DNA fingerprint profiles. Ten S. enterica isolates were selected from meat and human cases with an identical rep-PCR profile of serovars Rissen (n=4), Weltevreden (n=4), and Stanley (n=2). We used long-read whole genome sequencing (WGS) on the MinION sequencing platform to type isolates and investigate in silico the presence of specific AMR genes. Antimicrobial susceptibility testing was tested by disk diffusion and gradient diffusion method to corroborate the AMR phenotype. Multidrug resistance and resistance to more than one antimicrobial agent were observed in eight and nine isolates, respectively. Resistance to colistin with an accompanying mcr-1 gene was observed among the Salmonella isolates. The analysis of core genome and whole genome MLST revealed that the Salmonella from meat and human salmonellosis were genetically related. Hence, it could be concluded that meat is one of the important sources for Salmonella infection in human.
AB - Salmonella spp. is an important foodborne pathogen associated with consumption of contaminated food, especially food of livestock origin. Antimicrobial resistance (AMR) in Salmonella has been reported globally and increasing AMR in food production is a major public health issue worldwide. The objective of this study was to describe the genetic relatedness among Salmonella enterica isolates, which displayed identical DNA fingerprint profiles. Ten S. enterica isolates were selected from meat and human cases with an identical rep-PCR profile of serovars Rissen (n=4), Weltevreden (n=4), and Stanley (n=2). We used long-read whole genome sequencing (WGS) on the MinION sequencing platform to type isolates and investigate in silico the presence of specific AMR genes. Antimicrobial susceptibility testing was tested by disk diffusion and gradient diffusion method to corroborate the AMR phenotype. Multidrug resistance and resistance to more than one antimicrobial agent were observed in eight and nine isolates, respectively. Resistance to colistin with an accompanying mcr-1 gene was observed among the Salmonella isolates. The analysis of core genome and whole genome MLST revealed that the Salmonella from meat and human salmonellosis were genetically related. Hence, it could be concluded that meat is one of the important sources for Salmonella infection in human.
KW - Antimicrobial resistance
KW - Long-read sequencing
KW - Salmonella enterica
KW - Salmonellosis
KW - Typing
KW - Whole genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85108685290&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2021.109314
DO - 10.1016/j.ijfoodmicro.2021.109314
M3 - Article
SN - 0168-1605
VL - 358
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
M1 - 109314
ER -
