Changes in Nanoscale Chain Assembly in Sweet Potato Starch Lamellae by Downregulation of Biosynthesis Enzymes

Binjia Zhang; Wenzhi Zhou; Dongling Qiao; Peng Zhang; Siming Zhao; Liang Zhang; Fengwei Xie

doi:10.1021/acs.jafc.8b06523

Changes in Nanoscale Chain Assembly in Sweet Potato Starch Lamellae by Downregulation of Biosynthesis Enzymes

Binjia Zhang, Wenzhi Zhou, Dongling Qiao, Peng Zhang, Siming Zhao, Liang Zhang, Fengwei Xie

Research output: Contribution to journal › Article › peer-review

26 Citations (SciVal)

Abstract

Granule-bound starch synthase I (GBSSI) and starch-branching enzymes I and II (SBEI and SBEII) are crucial enzymes that biosynthesize starches with varied apparent amylose contents and amylopectin branching structure. With a sweet potato (Ipomoea batatas [L.] Lam. cv. Xushu22), this work shows that downregulating GBSSI (for waxy starch) or SBE (for high-amylose starch) activity allowed the formation of new semicrystalline lamellae (named Type II) in sweet potato starch in addition to the widely reported Type I lamellae. Small-angle X-ray scattering (SAXS) results show that, compared with Type I lamellae, Type II lamellae displayed increased average thickness and thickness-distribution width, with thickened amorphous and crystalline components. The size-exclusion-chromatography (SEC) data revealed mainly two enzyme sets, (i) and (ii), synthesizing amylopectin chains. Reducing the GBSSI or SBE activity increased the amounts of amylopectin long chains (degree of polymerization (DP) ≥ 33). Combined SAXS and SEC analyses indicate that parts of these long chains from enzyme set (i) could be confined to Type II lamellae, followed by DP ≤ 32 short chains in Type I lamellae and the rest of the long chains from enzyme sets (i) and (ii) spanning more than a single lamella.

Original language	English
Pages (from-to)	6302-6312
Number of pages	11
Journal	Journal of Agricultural and Food Chemistry
Volume	67
Issue number	22
DOIs	https://doi.org/10.1021/acs.jafc.8b06523
Publication status	Published - 5 Jun 2019

Funding

The authors would like to acknowledge the National Natural Science Foundation of China (NSFC, Project Nos. 31701637 and 31801582), the Fundamental Research Funds for the Central Universities (Project No. 2662016QD008), the Chinese Academy of Sciences (Project No. XDPB04), and a project funded by the China Postdoctoral Science Foundation (Project No. 2018M642865). B.Z. thanks the Young Elite Scientists Sponsorship Program of the China Association for Science and Technology, the Chutian Sholars Program of Hubei Province, and the Shishan Sholars Program of Huazhong Agricultural University. F.X. acknowledges the support of the European Union’s Marie Skłodowska-Curie Actions (MSCA) and the Institute of Advanced Study (IAS), University of Warwick, for the Warwick Interdisciplinary Research Leadership Programme (WIRL-COFUND). The authors also thank Dr. Cheng Li, Dr. Enpeng Li, and Shiqing Zhou from Professor Robert G. Gilbert’s lab at Yangzhou University for their assistance on SEC analysis.

Funders	Funder number
Chutian Sholars Program of Hubei Province
European Union’s Marie Skłodowska-Curie Actions
Institute of Advanced Study
Shishan Sholars Program of Huazhong Agricultural University
China Association for Science and Technology
Horizon 2020 Framework Programme	713548
H2020 Marie Skłodowska-Curie Actions
University of Warwick
National Natural Science Foundation of China	31701637, 31801582
Chinese Academy of Sciences	XDPB04
China Postdoctoral Science Foundation	2018M642865
Fundamental Research Funds for the Central Universities	2662016QD008

Keywords

molecular chain features
semicrystalline lamellae
starch
X-ray scattering

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/acs.jafc.8b06523

Cite this

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title = "Changes in Nanoscale Chain Assembly in Sweet Potato Starch Lamellae by Downregulation of Biosynthesis Enzymes",

abstract = "Granule-bound starch synthase I (GBSSI) and starch-branching enzymes I and II (SBEI and SBEII) are crucial enzymes that biosynthesize starches with varied apparent amylose contents and amylopectin branching structure. With a sweet potato (Ipomoea batatas [L.] Lam. cv. Xushu22), this work shows that downregulating GBSSI (for waxy starch) or SBE (for high-amylose starch) activity allowed the formation of new semicrystalline lamellae (named Type II) in sweet potato starch in addition to the widely reported Type I lamellae. Small-angle X-ray scattering (SAXS) results show that, compared with Type I lamellae, Type II lamellae displayed increased average thickness and thickness-distribution width, with thickened amorphous and crystalline components. The size-exclusion-chromatography (SEC) data revealed mainly two enzyme sets, (i) and (ii), synthesizing amylopectin chains. Reducing the GBSSI or SBE activity increased the amounts of amylopectin long chains (degree of polymerization (DP) ≥ 33). Combined SAXS and SEC analyses indicate that parts of these long chains from enzyme set (i) could be confined to Type II lamellae, followed by DP ≤ 32 short chains in Type I lamellae and the rest of the long chains from enzyme sets (i) and (ii) spanning more than a single lamella.",

keywords = "molecular chain features, semicrystalline lamellae, starch, X-ray scattering",

author = "Binjia Zhang and Wenzhi Zhou and Dongling Qiao and Peng Zhang and Siming Zhao and Liang Zhang and Fengwei Xie",

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T1 - Changes in Nanoscale Chain Assembly in Sweet Potato Starch Lamellae by Downregulation of Biosynthesis Enzymes

AU - Zhang, Binjia

AU - Zhou, Wenzhi

AU - Qiao, Dongling

AU - Zhang, Peng

AU - Zhao, Siming

AU - Zhang, Liang

AU - Xie, Fengwei

PY - 2019/6/5

Y1 - 2019/6/5

N2 - Granule-bound starch synthase I (GBSSI) and starch-branching enzymes I and II (SBEI and SBEII) are crucial enzymes that biosynthesize starches with varied apparent amylose contents and amylopectin branching structure. With a sweet potato (Ipomoea batatas [L.] Lam. cv. Xushu22), this work shows that downregulating GBSSI (for waxy starch) or SBE (for high-amylose starch) activity allowed the formation of new semicrystalline lamellae (named Type II) in sweet potato starch in addition to the widely reported Type I lamellae. Small-angle X-ray scattering (SAXS) results show that, compared with Type I lamellae, Type II lamellae displayed increased average thickness and thickness-distribution width, with thickened amorphous and crystalline components. The size-exclusion-chromatography (SEC) data revealed mainly two enzyme sets, (i) and (ii), synthesizing amylopectin chains. Reducing the GBSSI or SBE activity increased the amounts of amylopectin long chains (degree of polymerization (DP) ≥ 33). Combined SAXS and SEC analyses indicate that parts of these long chains from enzyme set (i) could be confined to Type II lamellae, followed by DP ≤ 32 short chains in Type I lamellae and the rest of the long chains from enzyme sets (i) and (ii) spanning more than a single lamella.

AB - Granule-bound starch synthase I (GBSSI) and starch-branching enzymes I and II (SBEI and SBEII) are crucial enzymes that biosynthesize starches with varied apparent amylose contents and amylopectin branching structure. With a sweet potato (Ipomoea batatas [L.] Lam. cv. Xushu22), this work shows that downregulating GBSSI (for waxy starch) or SBE (for high-amylose starch) activity allowed the formation of new semicrystalline lamellae (named Type II) in sweet potato starch in addition to the widely reported Type I lamellae. Small-angle X-ray scattering (SAXS) results show that, compared with Type I lamellae, Type II lamellae displayed increased average thickness and thickness-distribution width, with thickened amorphous and crystalline components. The size-exclusion-chromatography (SEC) data revealed mainly two enzyme sets, (i) and (ii), synthesizing amylopectin chains. Reducing the GBSSI or SBE activity increased the amounts of amylopectin long chains (degree of polymerization (DP) ≥ 33). Combined SAXS and SEC analyses indicate that parts of these long chains from enzyme set (i) could be confined to Type II lamellae, followed by DP ≤ 32 short chains in Type I lamellae and the rest of the long chains from enzyme sets (i) and (ii) spanning more than a single lamella.

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Changes in Nanoscale Chain Assembly in Sweet Potato Starch Lamellae by Downregulation of Biosynthesis Enzymes

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