Cell wall degrading enzymes of vascular wilt fungi. III. Possible involvement of endo-pectin lyase in Verticillium wilt of tomato

Richard M Cooper

Research output: Contribution to journalArticle

Abstract

High endo-pectin lyase (endo-PL) activity occurred in vascular tissue of susceptible tomato cuttings (cv. Gem S.) 3 days after infection by Verticillium albo-atrum and before symptoms appeared. The enzyme also occurred in a near isogenic resistant cultivar (Gem R.) but at much lower levels. Endo-polygalacturonase (endo-PG) was also found but only at slightly above endogenous levels. Activities of both enzymes decreased markedly as symptoms developed.

In stem segments infiltrated with propagules of V. alto-atrum, endo-PL was the only cell wall-degrading enzyme with increased activity. Similar and high levels were detected after 3 to 4 days in vascular tissue excised from Gem R and Gem S stems, and were related to inoculum density. Calculation of volume of xylem fluids indicated that endo-PL activity in vessels of infected stems was comparable to that attained in cultures containing polygalacturonide substrates.

In root-inoculated plants (cv. Craigella) endo-PG increased slightly at first but later there was no increase in activity of any wall-degrading enzyme, although vessels contained gels and degraded pit membranes.

Disease symptoms, including vascular gels, were reproduced by supplying stems and leaves with partially purified endo-PL and endo-PG so that they contained amounts similar to those detected in xylem of infected plants.

The significance of enzymic degradation or alteration of pit membranes is discussed in relation to the induction of water stress.

Original languageEnglish
Pages (from-to)285-300
Number of pages16
JournalPhysiologial Plant Pathology
Volume16
Issue number2
DOIs
Publication statusPublished - 1980

Fingerprint

pectin lyase
vascular wilt
Verticillium wilt
polygalacturonase
cell walls
tomatoes
signs and symptoms (plants)
fungi
stems
xylem vessels
vascular tissues
enzymes
xylem
gels
Verticillium albo-atrum
inoculum density
blood vessels
water stress
enzyme activity
degradation

Cite this

Cell wall degrading enzymes of vascular wilt fungi. III. Possible involvement of endo-pectin lyase in Verticillium wilt of tomato. / Cooper, Richard M.

In: Physiologial Plant Pathology, Vol. 16, No. 2, 1980, p. 285-300.

Research output: Contribution to journalArticle

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abstract = "High endo-pectin lyase (endo-PL) activity occurred in vascular tissue of susceptible tomato cuttings (cv. Gem S.) 3 days after infection by Verticillium albo-atrum and before symptoms appeared. The enzyme also occurred in a near isogenic resistant cultivar (Gem R.) but at much lower levels. Endo-polygalacturonase (endo-PG) was also found but only at slightly above endogenous levels. Activities of both enzymes decreased markedly as symptoms developed. In stem segments infiltrated with propagules of V. alto-atrum, endo-PL was the only cell wall-degrading enzyme with increased activity. Similar and high levels were detected after 3 to 4 days in vascular tissue excised from Gem R and Gem S stems, and were related to inoculum density. Calculation of volume of xylem fluids indicated that endo-PL activity in vessels of infected stems was comparable to that attained in cultures containing polygalacturonide substrates. In root-inoculated plants (cv. Craigella) endo-PG increased slightly at first but later there was no increase in activity of any wall-degrading enzyme, although vessels contained gels and degraded pit membranes. Disease symptoms, including vascular gels, were reproduced by supplying stems and leaves with partially purified endo-PL and endo-PG so that they contained amounts similar to those detected in xylem of infected plants. The significance of enzymic degradation or alteration of pit membranes is discussed in relation to the induction of water stress.",
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AB - High endo-pectin lyase (endo-PL) activity occurred in vascular tissue of susceptible tomato cuttings (cv. Gem S.) 3 days after infection by Verticillium albo-atrum and before symptoms appeared. The enzyme also occurred in a near isogenic resistant cultivar (Gem R.) but at much lower levels. Endo-polygalacturonase (endo-PG) was also found but only at slightly above endogenous levels. Activities of both enzymes decreased markedly as symptoms developed. In stem segments infiltrated with propagules of V. alto-atrum, endo-PL was the only cell wall-degrading enzyme with increased activity. Similar and high levels were detected after 3 to 4 days in vascular tissue excised from Gem R and Gem S stems, and were related to inoculum density. Calculation of volume of xylem fluids indicated that endo-PL activity in vessels of infected stems was comparable to that attained in cultures containing polygalacturonide substrates. In root-inoculated plants (cv. Craigella) endo-PG increased slightly at first but later there was no increase in activity of any wall-degrading enzyme, although vessels contained gels and degraded pit membranes. Disease symptoms, including vascular gels, were reproduced by supplying stems and leaves with partially purified endo-PL and endo-PG so that they contained amounts similar to those detected in xylem of infected plants. The significance of enzymic degradation or alteration of pit membranes is discussed in relation to the induction of water stress.

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