cDNA cloning of eel (Anguilla anguilla) somatolactin

Denise May, Christopher M. Todd, Mariann Rand-Weaver

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The nucleotide sequence of the cDNA coding for eel somatolactin (SL), a pituitary hormone belonging to the growth hormone (GH)/prolactin (PRL) family, has been determined. The full-length eel SL cDNA (1213 bp) encodes a protein of 229 amino acids (aa), with a putative signal peptide of 24 aa and a mature protein of 204 aa. Eel SL contains seven Cys residues found to be characteristic of SLs, and two potential N-glycosylation sites. Significant sequence homology between eel and seven other fish SLs (42% aa identity, 63% aa similarity) reveal SL to be highly conserved. A higher sequence identity of SL to GH than PRL is suggested by the comparison of these hormones in eel and chum salmon.
Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalGene
Volume188
Issue number1
DOIs
Publication statusPublished - 25 Mar 1997

Fingerprint

Anguilla
Eels
Organism Cloning
Complementary DNA
Amino Acids
Prolactin
Growth Hormone
Oncorhynchus keta
Pituitary Hormones
Sequence Homology
Protein Sorting Signals
Glycosylation
Fishes
Proteins
Hormones

Keywords

  • Amino acid sequence homology
  • Fish
  • Growth hormone-prolactin family
  • Nucleotide sequencing
  • Pituitary
  • complementary DNA
  • hypophysis hormone
  • signal peptide
  • somatolactin
  • unclassified drug
  • amino acid sequence
  • animal cell
  • article
  • eel
  • fish
  • molecular cloning
  • nonhuman
  • Northern blotting
  • nucleotide sequence
  • priority journal
  • protein expression
  • protein glycosylation
  • sequence homology

Cite this

cDNA cloning of eel (Anguilla anguilla) somatolactin. / May, Denise; Todd, Christopher M.; Rand-Weaver, Mariann.

In: Gene, Vol. 188, No. 1, 25.03.1997, p. 63-67.

Research output: Contribution to journalArticle

May, Denise ; Todd, Christopher M. ; Rand-Weaver, Mariann. / cDNA cloning of eel (Anguilla anguilla) somatolactin. In: Gene. 1997 ; Vol. 188, No. 1. pp. 63-67.
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abstract = "The nucleotide sequence of the cDNA coding for eel somatolactin (SL), a pituitary hormone belonging to the growth hormone (GH)/prolactin (PRL) family, has been determined. The full-length eel SL cDNA (1213 bp) encodes a protein of 229 amino acids (aa), with a putative signal peptide of 24 aa and a mature protein of 204 aa. Eel SL contains seven Cys residues found to be characteristic of SLs, and two potential N-glycosylation sites. Significant sequence homology between eel and seven other fish SLs (42{\%} aa identity, 63{\%} aa similarity) reveal SL to be highly conserved. A higher sequence identity of SL to GH than PRL is suggested by the comparison of these hormones in eel and chum salmon.",
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