Abstract
The nucleotide sequence of the cDNA coding for eel somatolactin (SL), a pituitary hormone belonging to the growth hormone (GH)/prolactin (PRL) family, has been determined. The full-length eel SL cDNA (1213 bp) encodes a protein of 229 amino acids (aa), with a putative signal peptide of 24 aa and a mature protein of 204 aa. Eel SL contains seven Cys residues found to be characteristic of SLs, and two potential N-glycosylation sites. Significant sequence homology between eel and seven other fish SLs (42% aa identity, 63% aa similarity) reveal SL to be highly conserved. A higher sequence identity of SL to GH than PRL is suggested by the comparison of these hormones in eel and chum salmon.
| Original language | English |
|---|---|
| Pages (from-to) | 63-67 |
| Number of pages | 5 |
| Journal | Gene |
| Volume | 188 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 25 Mar 1997 |
Keywords
- Amino acid sequence homology
- Fish
- Growth hormone-prolactin family
- Nucleotide sequencing
- Pituitary
- complementary DNA
- hypophysis hormone
- signal peptide
- somatolactin
- unclassified drug
- amino acid sequence
- animal cell
- article
- eel
- fish
- molecular cloning
- nonhuman
- Northern blotting
- nucleotide sequence
- priority journal
- protein expression
- protein glycosylation
- sequence homology