CD38 Structure-Based Inhibitor Design Using the N1-Cyclic Inosine 5′-Diphosphate Ribose Template

C. Moreau, Q. Liu, R. Graeff, G.K. Wagner, M.P. Thomas, J.M. Swarbrick, S. Shuto, H.C. Lee, Q. Hao, B.V.L. Potter

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Abstract

Few inhibitors exist for CD38, a multifunctional enzyme catalyzing the formation and metabolism of the Ca-mobilizing second messenger cyclic adenosine 5′-diphosphoribose (cADPR). Synthetic, non-hydrolyzable ligands can facilitate structure-based inhibitor design. Molecular docking was used to reproduce the crystallographic binding mode of cyclic inosine 5′-diphosphoribose (N1-cIDPR) with CD38, revealing an exploitable pocket and predicting the potential to introduce an extra hydrogen bond interaction with Asp-155. The purine C-8 position of N1-cIDPR (IC 276 μM) was extended with an amino or diaminobutane group and the 8-modified compounds were evaluated against CD38-catalyzed cADPR hydrolysis. Crystallography of an 8-amino N1-cIDPR:CD38 complex confirmed the predicted interaction with Asp-155, together with a second H-bond from a realigned Glu-146, rationalizing the improved inhibition (IC 56 μM). Crystallography of a complex of cyclic ADP-carbocyclic ribose (cADPcR, IC 129 μM) with CD38 illustrated that Glu-146 hydrogen bonds with the ligand N6-amino group. Both 8-amino N1-cIDPR and cADPcR bind deep in the active site reaching the catalytic residue Glu-226, and mimicking the likely location of cADPR during catalysis. Substantial overlap of the N1-cIDPR "northern" ribose monophosphate and the cADPcR carbocyclic ribose monophosphate regions suggests that this area is crucial for inhibitor design, leading to a new compound series of N1-inosine 5′-monophosphates (N1-IMPs). These small fragments inhibit hydrolysis of cADPR more efficiently than the parent cyclic compounds, with the best in the series demonstrating potent inhibition (IC = 7.6 μM). The lower molecular weight and relative simplicity of these compounds compared to cADPR make them attractive as a starting point for further inhibitor design.
Original languageEnglish
Article numbere66247
Number of pages15
JournalPLoS ONE
Volume8
Issue number6
DOIs
Publication statusPublished - 19 Jun 2013

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    Moreau, C., Liu, Q., Graeff, R., Wagner, G. K., Thomas, M. P., Swarbrick, J. M., Shuto, S., Lee, H. C., Hao, Q., & Potter, B. V. L. (2013). CD38 Structure-Based Inhibitor Design Using the N1-Cyclic Inosine 5′-Diphosphate Ribose Template. PLoS ONE, 8(6), [e66247]. https://doi.org/10.1371/journal.pone.0066247