Projects per year
Abstract
Despite increasing evidence to indicate that long non-coding RNAs (lncRNAs) are novel regulators of immunity, there has been no systematic attempt to identify and characterize the lncRNAs whose expression is changed following the induction of the innate immune response. To address this issue, we have employed next-generation sequencing data to determine the changes in the lncRNA profile in four human (monocytes, macrophages, epithelium, and chondrocytes) and four mouse cell types (RAW 264.7 macrophages, bone marrow-derived macrophages, peritoneal macrophages, and splenic dendritic cells) following exposure to the pro-inflammatory mediators, lipopolysaccharides (LPS), or interleukin-1β. We show differential expression of 204 human and 210 mouse lncRNAs, with positional analysis demonstrating correlation with immune-related genes. These lncRNAs are predominantly cell-type specific, composed of large regions of repeat sequences, and show poor evolutionary conservation. Comparison within the human and mouse sequences showed less than 1% sequence conservation, although we identified multiple conserved motifs. Of the 204 human lncRNAs, 21 overlapped with syntenic mouse lncRNAs, of which five were differentially expressed in both species. Among these syntenic lncRNA was IL7-AS (antisense), which was induced in multiple cell types and shown to regulate the production of the pro-inflammatory mediator interleukin-6 in both human and mouse cells. In summary, we have identified and characterized those lncRNAs that are differentially expressed following activation of the human and mouse innate immune responses and believe that these catalogs will provide the foundation for the future analysis of the role of lncRNAs in immune and inflammatory responses.
Original language | English |
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Article number | 1038 |
Number of pages | 20 |
Journal | Frontiers in Immunology |
Volume | 8 |
Early online date | 29 Aug 2017 |
DOIs | |
Publication status | Published - 31 Dec 2017 |
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Dive into the research topics of 'Catalog of Differentially Expressed Long Non-Coding RNA following Activation of Human and Mouse Innate Immune Response'. Together they form a unique fingerprint.Projects
- 2 Finished
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Elucidating the Role of Long Non-coding RNAs
Lindsay, M. (PI) & Hurst, L. (CoI)
Biotechnology and Biological Sciences Research Council
1/07/16 → 30/04/20
Project: Research council
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Are Long Intergenic Non-ccoding RNAs Central Regulators of Inflammation and the Innate Immune Response?
Lindsay, M. (PI)
Biotechnology and Biological Sciences Research Council
3/04/13 → 2/04/16
Project: Research council
Profiles
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Mark Lindsay
- Department of Life Sciences - Professor
- Centre for Therapeutic Innovation
- Centre for Bioengineering & Biomedical Technologies (CBio)
Person: Research & Teaching, Affiliate staff