Ca2+ entry induced by cyclic ADP-ribose in intact T-lymphocytes

Andreas H. Guse, Ingeborg Berg, Cristina P. da Silva, Barry V. L. Potter, Georg W. Mayr

Research output: Contribution to journalArticle

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Abstract

Cyclic ADP-ribose (cADPr) is a potent Ca2+-mobilizing natural compound (Lee, H. C., Walseth, T. F., Bratt, G. T., Hayes, R. N., and Clapper, D. L. (1989) J. Biol. Chem. 264, 1608-1615) which has been shown to release Ca2+ from an intracellular store of permeabilized T-lymphocytes (Guse, A. H., Silva, C. P., Emmrich, F., Ashamu, G., Potter, B. V. L., and Mayr, G. W. (1995) J. Immunol. 155, 3353-3359). Microinjection of cADPr into intact single T lymphocytes dose dependently induced repetitive but irregular Ca2+ spikes which were almost completely dependent on the presence of extracellular Ca2+. The Ca2+ spikes induced by cADPr could be blocked either by co-injection of cADPr with the specific antagonist 8-NH2-cADPr, by omission of Ca2+ from the medium, or by superfusion of the cells with Zn2+ or SK-F 96365. Ratiometric digital Ca2+ imaging revealed that single Ca2+ spikes were initiated at several sites (“hot spots”) close to the plasma membrane. These hot spots then rapidly formed a circular zone of high Ca2+ concentration below the plasma membrane which subsequently propagated like a closing optical diaphragm into the center of the cell. Taken together these data indicate a role for cADPr in Ca2+ entry in T-lymphocytes.
Original languageEnglish
Pages (from-to)8546-8550
JournalJournal of Biological Chemistry
Volume272
Issue number13
DOIs
Publication statusPublished - 28 Mar 1997

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Cyclic ADP-Ribose
T-cells
T-Lymphocytes
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Cell membranes
Cell Membrane
Microinjections
Diaphragms
Diaphragm
Imaging techniques
Injections

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Ca2+ entry induced by cyclic ADP-ribose in intact T-lymphocytes. / Guse, Andreas H.; Berg, Ingeborg; da Silva, Cristina P.; Potter, Barry V. L.; Mayr, Georg W.

In: Journal of Biological Chemistry, Vol. 272, No. 13, 28.03.1997, p. 8546-8550.

Research output: Contribution to journalArticle

Guse, Andreas H. ; Berg, Ingeborg ; da Silva, Cristina P. ; Potter, Barry V. L. ; Mayr, Georg W. / Ca2+ entry induced by cyclic ADP-ribose in intact T-lymphocytes. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 13. pp. 8546-8550.
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