Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation

K. Szot, A. de Poulpiquet, A. Ciaccafava, H. Marques, M. Jönsson-Niedziolka, J. Niedziolka-Jönsson, F. Marken, E. Lojou, M. Opallo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Hydrophilic carbon nanoparticles were utilized as an electroconductive and adsorbent material for membrane-bound [NiFe] hydrogenase obtained from Aquifex aeolicus. Herein, a bottom-up approach was utilized for the preparation of carbon nanoparticulate films on ITO electrodes. The films were prepared either from carbon nanoparticles of opposite charges using a layer-by-layer method, or from negatively charged carbon nanoparticles by their encapsulation in a hydrophobic sol-gel processed silicate matrix. The enzyme was adsorbed on the carbon nanoparticulate film and its catalytic activity towards hydrogen oxidation was studied by cyclic voltammetry and chronoamperometry. The adsorbed hydrogenase exhibits efficient mediatorless electrocatalysis with a maximum activity at temperatures ranging from 60 to 85°C. The effect on the bioelectrocatalytic hydrogen oxidation of adding a mediator to the solution or immobilized on the carbon nanoparticulate film electrodes was also studied.

Original languageEnglish
Pages (from-to)434-440
Number of pages7
JournalElectrochimica Acta
Volume111
DOIs
Publication statusPublished - 30 Nov 2013

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Carbon films
Scaffolds
Hydrogen
Carbon
Nanoparticles
Oxidation
Hydrogenase
Silicates
Electrocatalysis
Chronoamperometry
Electrodes
Encapsulation
Adsorbents
Cyclic voltammetry
Sol-gels
Catalyst activity
Thermodynamic properties
Enzymes
Membranes
Temperature

Cite this

Szot, K., de Poulpiquet, A., Ciaccafava, A., Marques, H., Jönsson-Niedziolka, M., Niedziolka-Jönsson, J., ... Opallo, M. (2013). Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation. Electrochimica Acta, 111, 434-440. https://doi.org/10.1016/j.electacta.2013.08.001

Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation. / Szot, K.; de Poulpiquet, A.; Ciaccafava, A.; Marques, H.; Jönsson-Niedziolka, M.; Niedziolka-Jönsson, J.; Marken, F.; Lojou, E.; Opallo, M.

In: Electrochimica Acta, Vol. 111, 30.11.2013, p. 434-440.

Research output: Contribution to journalArticle

Szot, K, de Poulpiquet, A, Ciaccafava, A, Marques, H, Jönsson-Niedziolka, M, Niedziolka-Jönsson, J, Marken, F, Lojou, E & Opallo, M 2013, 'Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation', Electrochimica Acta, vol. 111, pp. 434-440. https://doi.org/10.1016/j.electacta.2013.08.001
Szot K, de Poulpiquet A, Ciaccafava A, Marques H, Jönsson-Niedziolka M, Niedziolka-Jönsson J et al. Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation. Electrochimica Acta. 2013 Nov 30;111:434-440. https://doi.org/10.1016/j.electacta.2013.08.001
Szot, K. ; de Poulpiquet, A. ; Ciaccafava, A. ; Marques, H. ; Jönsson-Niedziolka, M. ; Niedziolka-Jönsson, J. ; Marken, F. ; Lojou, E. ; Opallo, M. / Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation. In: Electrochimica Acta. 2013 ; Vol. 111. pp. 434-440.
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