Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.
Li, Z., Deng, S-S., Zang, Y., Gu, Z., He, X-P., Chen, G-R., Chen, K., James, T. D., Li, J., & Long, Y-T. (2013). Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system. Scientific Reports, 3, . https://doi.org/10.1038/srep02293