Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system

Z. Li, S.-S. Deng, Y. Zang, Z. Gu, X.-P. He, G.-R. Chen, K. Chen, T.D. James, J. Li, Y.-T. Long

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Abstract

Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.
Original languageEnglish
Article number2293
Number of pages7
JournalScientific Reports
Volume3
DOIs
Publication statusPublished - 29 Jul 2013

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Sugars
Asialoglycoprotein Receptor
Ligands
Anthraquinones
Graphite
Biomimetics
Self assembly
Glycoproteins
Cells
Electrodes

Cite this

Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system. / Li, Z.; Deng, S.-S.; Zang, Y.; Gu, Z.; He, X.-P.; Chen, G.-R.; Chen, K.; James, T.D.; Li, J.; Long, Y.-T.

In: Scientific Reports, Vol. 3, 2293, 29.07.2013.

Research output: Contribution to journalArticle

Li, Z. ; Deng, S.-S. ; Zang, Y. ; Gu, Z. ; He, X.-P. ; Chen, G.-R. ; Chen, K. ; James, T.D. ; Li, J. ; Long, Y.-T. / Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system. In: Scientific Reports. 2013 ; Vol. 3.
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