Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system

Z. Li; S.-S. Deng; Y. Zang; Z. Gu; X.-P. He; G.-R. Chen; K. Chen; T.D. James; J. Li; Y.-T. Long

doi:10.1038/srep02293

Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system

Z. Li, S.-S. Deng, Y. Zang, Z. Gu, X.-P. He, G.-R. Chen, K. Chen, T.D. James, J. Li, Y.-T. Long

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Abstract

Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.

Original language	English
Article number	2293
Number of pages	7
Journal	Scientific Reports
Volume	3
DOIs	https://doi.org/10.1038/srep02293
Publication status	Published - 29 Jul 2013

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AU - Li, Z.

AU - Deng, S.-S.

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AU - Gu, Z.

AU - He, X.-P.

AU - Chen, G.-R.

AU - Chen, K.

AU - James, T.D.

AU - Li, J.

AU - Long, Y.-T.

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AB - Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.

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Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system

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