Capacitive malaria aptasensor using Plasmodium falciparum glutamate dehydrogenase as target antigen in undiluted human serum

Naveen Singh, Sunil Arya, Pedro Estrela, Pranab Goswami

Research output: Contribution to journalArticle

9 Citations (Scopus)
32 Downloads (Pure)

Abstract

A capacitive aptasensor for detecting the malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (PfGDH), directly in human serum samples developed. A thiolated ssDNA aptamer (NG3) that binds specifically to PfGDH antigen with high affinity (Kd= 79 nM) was used to develop the aptasensor. The aptasensor produced capacitance response at an optimized frequency of 2 Hz in a non-Faradaic electrochemical impedance based signal transduction platform. The aptasensor exhibited a wide dynamic range of 100 fM − 100 nM with a limits of detection of 0.77 pM in serum samples. The interference from other predominant malarial biomarkers, namely, Plasmodium falciparum –lactate dehydrogenase and -histidine rich protein-II on the aptasensor was negligible. This PfGDH aptasensor with highly sensitive and label free detection capability has great application potential for diagnosis of asymptotic malaria and monitoring the regression of malaria during treatment regime with antimalarial drugs.
Original languageEnglish
Pages (from-to)246-252
Number of pages7
JournalBiosensors and Bioelectronics
Volume117
Early online date8 Jun 2018
DOIs
Publication statusPublished - 15 Oct 2018

Keywords

  • Aptamer
  • Biosensor
  • Capacitance
  • Surface plasmon resonance
  • PfGDH
  • Malaria

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