Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 246-252 |
| Number of pages | 7 |
| Journal | Biosensors and Bioelectronics |
| Volume | 117 |
| Early online date | 8 Jun 2018 |
| DOIs | |
| Publication status | Published - 15 Oct 2018 |
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Keywords
- Aptamer
- Biosensor
- Capacitance
- Surface plasmon resonance
- PfGDH
- Malaria
Cite this
Capacitive malaria aptasensor using Plasmodium falciparum glutamate dehydrogenase as target antigen in undiluted human serum. / Singh, Naveen; Arya, Sunil; Estrela, Pedro; Goswami, Pranab.
In: Biosensors and Bioelectronics, Vol. 117, 15.10.2018, p. 246-252.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Capacitive malaria aptasensor using Plasmodium falciparum glutamate dehydrogenase as target antigen in undiluted human serum
AU - Singh, Naveen
AU - Arya, Sunil
AU - Estrela, Pedro
AU - Goswami, Pranab
PY - 2018/10/15
Y1 - 2018/10/15
N2 - A capacitive aptasensor for detecting the malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (PfGDH), directly in human serum samples developed. A thiolated ssDNA aptamer (NG3) that binds specifically to PfGDH antigen with high affinity (Kd= 79 nM) was used to develop the aptasensor. The aptasensor produced capacitance response at an optimized frequency of 2 Hz in a non-Faradaic electrochemical impedance based signal transduction platform. The aptasensor exhibited a wide dynamic range of 100 fM − 100 nM with a limits of detection of 0.77 pM in serum samples. The interference from other predominant malarial biomarkers, namely, Plasmodium falciparum –lactate dehydrogenase and -histidine rich protein-II on the aptasensor was negligible. This PfGDH aptasensor with highly sensitive and label free detection capability has great application potential for diagnosis of asymptotic malaria and monitoring the regression of malaria during treatment regime with antimalarial drugs.
AB - A capacitive aptasensor for detecting the malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (PfGDH), directly in human serum samples developed. A thiolated ssDNA aptamer (NG3) that binds specifically to PfGDH antigen with high affinity (Kd= 79 nM) was used to develop the aptasensor. The aptasensor produced capacitance response at an optimized frequency of 2 Hz in a non-Faradaic electrochemical impedance based signal transduction platform. The aptasensor exhibited a wide dynamic range of 100 fM − 100 nM with a limits of detection of 0.77 pM in serum samples. The interference from other predominant malarial biomarkers, namely, Plasmodium falciparum –lactate dehydrogenase and -histidine rich protein-II on the aptasensor was negligible. This PfGDH aptasensor with highly sensitive and label free detection capability has great application potential for diagnosis of asymptotic malaria and monitoring the regression of malaria during treatment regime with antimalarial drugs.
KW - Aptamer
KW - Biosensor
KW - Capacitance
KW - Surface plasmon resonance
KW - PfGDH
KW - Malaria
U2 - 10.1016/j.bios.2018.06.022
DO - 10.1016/j.bios.2018.06.022
M3 - Article
VL - 117
SP - 246
EP - 252
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -