Projects per year
Abstract
A capacitive aptasensor for detecting the malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (PfGDH), directly in human serum samples developed. A thiolated ssDNA aptamer (NG3) that binds specifically to PfGDH antigen with high affinity (Kd= 79 nM) was used to develop the aptasensor. The aptasensor produced capacitance response at an optimized frequency of 2 Hz in a non-Faradaic electrochemical impedance based signal transduction platform. The aptasensor exhibited a wide dynamic range of 100 fM − 100 nM with a limits of detection of 0.77 pM in serum samples. The interference from other predominant malarial biomarkers, namely, Plasmodium falciparum –lactate dehydrogenase and -histidine rich protein-II on the aptasensor was negligible. This PfGDH aptasensor with highly sensitive and label free detection capability has great application potential for diagnosis of asymptotic malaria and monitoring the regression of malaria during treatment regime with antimalarial drugs.
Original language | English |
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Pages (from-to) | 246-252 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 117 |
Early online date | 8 Jun 2018 |
DOIs | |
Publication status | Published - 15 Oct 2018 |
Keywords
- Aptamer
- Biosensor
- Capacitance
- Surface plasmon resonance
- PfGDH
- Malaria
Fingerprint
Dive into the research topics of 'Capacitive malaria aptasensor using Plasmodium falciparum glutamate dehydrogenase as target antigen in undiluted human serum'. Together they form a unique fingerprint.Projects
- 1 Finished
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Marie Curie IF - Dr Sunil Kumar Arya - BlaC E-assay
Estrela, P. (PI)
5/10/15 → 4/10/17
Project: EU Commission
Profiles
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Pedro Estrela
- Department of Electronic & Electrical Engineering - Professor
- Centre for Bioengineering & Biomedical Technologies (CBio) - Centre Director
- Centre for Therapeutic Innovation
- Centre for Sustainable Chemical Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Centre for Nanoscience and Nanotechnology
- Institute of Sustainability and Climate Change
- Centre for Integrated Materials, Processes & Structures (IMPS)
- Centre for Regenerative Design & Engineering for a Net Positive World (RENEW)
- Bath Institute for the Augmented Human
- Centre of Excellence in Water-Based Early-Warning Systems for Health Protection (CWBE)
Person: Research & Teaching, Core staff, Affiliate staff
Equipment
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MC2- Surface analysis and spectroscopy
Material and Chemical Characterisation (MC2)Facility/equipment: Technology type