Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum

Sunil Arya, Pavel Zhurauski, Pawan Jolly, Marina Batistuti, Marcelo Mulato, Pedro Estrela

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Abstract

We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker. The sensor was characterized using cyclic voltammetry, electrochemical impedance spectroscopy (EIS), atomic force microscopy and contact angle studies. Non-Faradic EIS measurements were utilized to investigate the sensor performance via monitoring of the changes in capacitance. The aptasensor exhibited logarithmically detection of HER2 from 1 pM to 100 nM in both buffer and undiluted serum with limits of detection lower than 1 pM. The results pave the way to develop other aptamer-based biosensors for protein biomarkers detection in undiluted serum.
Original languageEnglish
Pages (from-to)106-112
Number of pages7
JournalBiosensors and Bioelectronics
Volume102
Early online date3 Nov 2017
DOIs
Publication statusPublished - 15 Apr 2018

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Keywords

  • Aptamer
  • Impedance
  • capacitance
  • Biosensor
  • HER2
  • Breast cancer

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