Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum

Sunil Arya, Pavel Zhurauski, Pawan Jolly, Marina Batistuti, Marcelo Mulato, Pedro Estrela

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Abstract

We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker. The sensor was characterized using cyclic voltammetry, electrochemical impedance spectroscopy (EIS), atomic force microscopy and contact angle studies. Non-Faradic EIS measurements were utilized to investigate the sensor performance via monitoring of the changes in capacitance. The aptasensor exhibited logarithmically detection of HER2 from 1 pM to 100 nM in both buffer and undiluted serum with limits of detection lower than 1 pM. The results pave the way to develop other aptamer-based biosensors for protein biomarkers detection in undiluted serum.
Original languageEnglish
Pages (from-to)106-112
Number of pages7
JournalBiosensors and Bioelectronics
Volume102
Early online date3 Nov 2017
DOIs
Publication statusPublished - 15 Apr 2018

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Dielectric Spectroscopy
Electrodes
Biomarkers
Breast Neoplasms
Electrochemical impedance spectroscopy
Gold
Buffers
Serum
Nucleotide Aptamers
Atomic Force Microscopy
Sensors
Biosensing Techniques
Sulfhydryl Compounds
Biosensors
Self assembly
Cyclic voltammetry
Contact angle
Limit of Detection
Atomic force microscopy
Phosphates

Keywords

  • Aptamer
  • Impedance
  • capacitance
  • Biosensor
  • HER2
  • Breast cancer

Cite this

Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum. / Arya, Sunil; Zhurauski, Pavel; Jolly, Pawan; Batistuti, Marina; Mulato, Marcelo; Estrela, Pedro.

In: Biosensors and Bioelectronics, Vol. 102, 15.04.2018, p. 106-112.

Research output: Contribution to journalArticle

Arya, Sunil ; Zhurauski, Pavel ; Jolly, Pawan ; Batistuti, Marina ; Mulato, Marcelo ; Estrela, Pedro. / Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum. In: Biosensors and Bioelectronics. 2018 ; Vol. 102. pp. 106-112.
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