Projects per year
Abstract
We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker. The sensor was characterized using cyclic voltammetry, electrochemical impedance spectroscopy (EIS), atomic force microscopy and contact angle studies. Non-Faradic EIS measurements were utilized to investigate the sensor performance via monitoring of the changes in capacitance. The aptasensor exhibited logarithmically detection of HER2 from 1 pM to 100 nM in both buffer and undiluted serum with limits of detection lower than 1 pM. The results pave the way to develop other aptamer-based biosensors for protein biomarkers detection in undiluted serum.
Original language | English |
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Pages (from-to) | 106-112 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 102 |
Early online date | 3 Nov 2017 |
DOIs | |
Publication status | Published - 15 Apr 2018 |
Keywords
- Aptamer
- Impedance
- capacitance
- Biosensor
- HER2
- Breast cancer
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Projects
- 3 Finished
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Cancer Diagnosis: Parallel Sensing of Prostate Cancer Biomarkers: MARIE CURIE - PROSENSE Training budget
Estrela, P., Eggleston, I., Frost, C., Lloyd, M., Pascu, S. & Tyrrell, R.
1/10/12 → 30/09/16
Project: EU Commission
Profiles
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Pedro Estrela
- Department of Electronic & Electrical Engineering - Senior Lecturer
- Centre for Biosensors, Bioelectronics and Biodevices (C3Bio)
- Centre for Therapeutic Innovation
- Centre for Sustainable and Circular Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Centre for Nanoscience and Nanotechnology
Person: Research & Teaching