Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 106-112 |
| Number of pages | 7 |
| Journal | Biosensors and Bioelectronics |
| Volume | 102 |
| Early online date | 3 Nov 2017 |
| DOIs | |
| Publication status | Published - 15 Apr 2018 |
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Keywords
- Aptamer
- Impedance
- capacitance
- Biosensor
- HER2
- Breast cancer
Cite this
Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum. / Arya, Sunil; Zhurauski, Pavel; Jolly, Pawan; Batistuti, Marina; Mulato, Marcelo; Estrela, Pedro.
In: Biosensors and Bioelectronics, Vol. 102, 15.04.2018, p. 106-112.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum
AU - Arya, Sunil
AU - Zhurauski, Pavel
AU - Jolly, Pawan
AU - Batistuti, Marina
AU - Mulato, Marcelo
AU - Estrela, Pedro
PY - 2018/4/15
Y1 - 2018/4/15
N2 - We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker. The sensor was characterized using cyclic voltammetry, electrochemical impedance spectroscopy (EIS), atomic force microscopy and contact angle studies. Non-Faradic EIS measurements were utilized to investigate the sensor performance via monitoring of the changes in capacitance. The aptasensor exhibited logarithmically detection of HER2 from 1 pM to 100 nM in both buffer and undiluted serum with limits of detection lower than 1 pM. The results pave the way to develop other aptamer-based biosensors for protein biomarkers detection in undiluted serum.
AB - We report the development of a simple and powerful capacitive aptasensor for the detection and estimation of human epidermal growth factor receptor 2 (HER2), a biomarker for breast cancer, in undiluted serum. The study involves the incorporation of interdigitated gold electrodes, which were used to prepare the electrochemical platform. A thiol terminated DNA aptamer with affinity for HER2 was used to prepare the bio-recognition layer via self-assembly on interdigitated gold surfaces. Non-specific binding was prevented by blocking free spaces on surface via starting block phosphate buffer saline-tween20 blocker. The sensor was characterized using cyclic voltammetry, electrochemical impedance spectroscopy (EIS), atomic force microscopy and contact angle studies. Non-Faradic EIS measurements were utilized to investigate the sensor performance via monitoring of the changes in capacitance. The aptasensor exhibited logarithmically detection of HER2 from 1 pM to 100 nM in both buffer and undiluted serum with limits of detection lower than 1 pM. The results pave the way to develop other aptamer-based biosensors for protein biomarkers detection in undiluted serum.
KW - Aptamer
KW - Impedance
KW - capacitance
KW - Biosensor
KW - HER2
KW - Breast cancer
U2 - 10.1016/j.bios.2017.11.013
DO - 10.1016/j.bios.2017.11.013
M3 - Article
VL - 102
SP - 106
EP - 112
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -