A simple probe for the detection of peroxynitrite was developed incorporating a benzyl boronic ester “protecting” unit. The “protecting” unit of the probe is removed by peroxynitrite to “turn-on” ESIPT fluorescence (4.5 fold enhancement). Furthermore, the probe was cell permeable and was used in cell imaging experiments showing an off–on response towards peroxynitrite, in HeLa and RAW 264.7 cells.
Sedgwick, A., Sun, X., Kim, G., Yoon, J., Bull, S. D., & James, T. D. (2016). Boronate based fluorescence (ESIPT) probe for peroxynitrite. Chemical Communications, 52(83), 12350-12352. https://doi.org/10.1039/C6CC06829D