Boronate based fluorescence (ESIPT) probe for peroxynitrite

Adam Sedgwick, Xiaolong Sun, Gyoungmi Kim, Juyoung Yoon, Steven D. Bull, Tony D. James

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A simple probe for the detection of peroxynitrite was developed incorporating a benzyl boronic ester “protecting” unit. The “protecting” unit of the probe is removed by peroxynitrite to “turn-on” ESIPT fluorescence (4.5 fold enhancement). Furthermore, the probe was cell permeable and was used in cell imaging experiments showing an off–on response towards peroxynitrite, in HeLa and RAW 264.7 cells.
Original languageEnglish
Pages (from-to)12350-12352
JournalChemical Communications
Volume52
Issue number83
Early online date19 Sep 2016
DOIs
Publication statusPublished - 25 Oct 2016

Fingerprint

Peroxynitrous Acid
Fluorescence
Esters
Imaging techniques
Experiments

Cite this

Boronate based fluorescence (ESIPT) probe for peroxynitrite. / Sedgwick, Adam; Sun, Xiaolong; Kim, Gyoungmi ; Yoon, Juyoung; Bull, Steven D.; James, Tony D.

In: Chemical Communications, Vol. 52, No. 83, 25.10.2016, p. 12350-12352.

Research output: Contribution to journalArticle

Sedgwick, Adam ; Sun, Xiaolong ; Kim, Gyoungmi ; Yoon, Juyoung ; Bull, Steven D. ; James, Tony D. / Boronate based fluorescence (ESIPT) probe for peroxynitrite. In: Chemical Communications. 2016 ; Vol. 52, No. 83. pp. 12350-12352.
@article{92aec42ab15c474da991fa0964924d53,
title = "Boronate based fluorescence (ESIPT) probe for peroxynitrite",
abstract = "A simple probe for the detection of peroxynitrite was developed incorporating a benzyl boronic ester “protecting” unit. The “protecting” unit of the probe is removed by peroxynitrite to “turn-on” ESIPT fluorescence (4.5 fold enhancement). Furthermore, the probe was cell permeable and was used in cell imaging experiments showing an off–on response towards peroxynitrite, in HeLa and RAW 264.7 cells.",
author = "Adam Sedgwick and Xiaolong Sun and Gyoungmi Kim and Juyoung Yoon and Bull, {Steven D.} and James, {Tony D.}",
year = "2016",
month = "10",
day = "25",
doi = "10.1039/C6CC06829D",
language = "English",
volume = "52",
pages = "12350--12352",
journal = "Chemical communications (Cambridge, England)",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "83",

}

TY - JOUR

T1 - Boronate based fluorescence (ESIPT) probe for peroxynitrite

AU - Sedgwick, Adam

AU - Sun, Xiaolong

AU - Kim, Gyoungmi

AU - Yoon, Juyoung

AU - Bull, Steven D.

AU - James, Tony D.

PY - 2016/10/25

Y1 - 2016/10/25

N2 - A simple probe for the detection of peroxynitrite was developed incorporating a benzyl boronic ester “protecting” unit. The “protecting” unit of the probe is removed by peroxynitrite to “turn-on” ESIPT fluorescence (4.5 fold enhancement). Furthermore, the probe was cell permeable and was used in cell imaging experiments showing an off–on response towards peroxynitrite, in HeLa and RAW 264.7 cells.

AB - A simple probe for the detection of peroxynitrite was developed incorporating a benzyl boronic ester “protecting” unit. The “protecting” unit of the probe is removed by peroxynitrite to “turn-on” ESIPT fluorescence (4.5 fold enhancement). Furthermore, the probe was cell permeable and was used in cell imaging experiments showing an off–on response towards peroxynitrite, in HeLa and RAW 264.7 cells.

U2 - 10.1039/C6CC06829D

DO - 10.1039/C6CC06829D

M3 - Article

VL - 52

SP - 12350

EP - 12352

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

SN - 1359-7345

IS - 83

ER -