Biotinylated boronic acid fluorophore conjugates: quencher elimination strategy for imaging and saccharide detection

Francois D'Hooge, Souad A Elfeky, Stephen Flower, Sofia I Pascu, A Toby A Jenkins, Johannes van den Elsen, Tony James, John Fossey

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18 Citations (Scopus)

Abstract

A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.
Original languageEnglish
Pages (from-to)3274-3280
Number of pages7
JournalRSC Advances
Volume2
DOIs
Publication statusPublished - 2012

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Boronic Acids
Fluorophores
Fluorescence microscopy
Microspheres
Esters
Fluorescence
Imaging techniques
Acids

Cite this

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title = "Biotinylated boronic acid fluorophore conjugates: quencher elimination strategy for imaging and saccharide detection",
abstract = "A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.",
author = "Francois D'Hooge and Elfeky, {Souad A} and Stephen Flower and Pascu, {Sofia I} and Jenkins, {A Toby A} and {van den Elsen}, Johannes and Tony James and John Fossey",
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AU - Elfeky, Souad A

AU - Flower, Stephen

AU - Pascu, Sofia I

AU - Jenkins, A Toby A

AU - van den Elsen, Johannes

AU - James, Tony

AU - Fossey, John

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