Abstract
Over the past two decades, bioorthogonal chemistry has undergone a remarkable development, challenging traditional assumptions in biology and medicine. Recent advancements in the design of probes tailored for bioorthogonal applications have met the increasing demand for precise imaging, facilitating the exploration of complex biological systems. These state-of-the-art probes enable highly sensitive, low background, in situ imaging of biological species and events within live organisms, achieving resolutions comparable to the size of the biomolecule under investigation. This review provides a comprehensive examination of various categories of bioorthogonally activated in situ fluorescent labels. It highlights the intricate design and benefits of bioorthogonal chemistry for precise in situ imaging, while also discussing future prospects in this rapidly evolving field.
| Original language | English |
|---|---|
| Pages (from-to) | 201-265 |
| Number of pages | 65 |
| Journal | Chemical Society Reviews |
| Volume | 54 |
| Issue number | 1 |
| Early online date | 18 Nov 2024 |
| DOIs | |
| Publication status | Published - 7 Jan 2025 |
Data Availability Statement
Data sharing is not applicable to this article as no new data were created or analyzed in this study.Acknowledgements
Facilities and assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis (CMM) and Queensland Node of the Australian National Fabrication Facility (ANFF-Q), the University of Queensland are also acknowledged.Funding
The authors gratefully acknowledge the financial support from the National Health and Medical Research Council (APP1175808). Y. F. is thankful for the generous financial support from the Natural Science Foundation of Jiangsu Province (Grant no. BK20240660). T. D. J. wishes to thank the University of Bath and the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University (2020ZD01) for support. Facilities and assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis (CMM) and Queensland Node of the Australian National Fabrication Facility (ANFF-Q), the University of Queensland are also acknowledged.
| Funders | Funder number |
|---|---|
| University of Bath | |
| National Health and Medical Research Council | APP1175808 |
| National Health and Medical Research Council | |
| Natural Science Foundation of Jiangsu Province | BK20240660 |
| Natural Science Foundation of Jiangsu Province | |
| Henan Normal University | 2020ZD01 |
| Henan Normal University |