Biomolecular and electrochemical charge detection by a micromechanical electrometer

Andreas Menzel, Angel T -H Lin, Pedro Estrela, Peng Li, Ashwin A Seshia

Research output: Contribution to journalArticle

7 Citations (Scopus)
76 Downloads (Pure)

Abstract

A micromechanical electrometer is applied to the label-free detection of biomolecular interactions and electrochemical charge sensing. The primary element of the electrometer is a micromechanical variable capacitor to modulate and convert a dc charge to an ac voltage output, thereby limiting the effects of low frequency noise on charge detection. At room temperature and ambient pressure the noise-limited charge resolution of a micromechanical electrometer based on this principle is found to be 3 e/ sqrt(Hz), enabling the potential detection of charged single molecule binding on electrode surfaces. The detection principle is validated by several experiments. Biomolecular binding experiments are conducted on an external gold electrode situated within a custom-designed flow cell and electrically connected to the micromachined electrometer. The concepts are validated by demonstrating the detection of biotin-streptavidin binding and DNA hybridization. Furthermore, it is shown that the electrometer can be applied for the detection of the redox system ferrocyanide/ferricyanide to describe Nernstian behaviour due to well defined charge transfer on the electrode surface at different concentration ratios as expected.
Original languageEnglish
Pages (from-to)301-305
Number of pages5
JournalSensors and Actuators B-Chemical
Volume160
Issue number1
DOIs
Publication statusPublished - 15 Dec 2011

Fingerprint

Electrometers
electrometers
Electrodes
electrodes
Streptavidin
Biotin
Gold
biotin
Charge transfer
Labels
DNA
Capacitors
Experiments
Molecules
capacitors
deoxyribonucleic acid
Electric potential
charge transfer
gold
low frequencies

Cite this

Biomolecular and electrochemical charge detection by a micromechanical electrometer. / Menzel, Andreas; Lin, Angel T -H; Estrela, Pedro; Li, Peng; Seshia, Ashwin A.

In: Sensors and Actuators B-Chemical, Vol. 160, No. 1, 15.12.2011, p. 301-305.

Research output: Contribution to journalArticle

Menzel, Andreas ; Lin, Angel T -H ; Estrela, Pedro ; Li, Peng ; Seshia, Ashwin A. / Biomolecular and electrochemical charge detection by a micromechanical electrometer. In: Sensors and Actuators B-Chemical. 2011 ; Vol. 160, No. 1. pp. 301-305.
@article{be2e56e2832e4b7a8a2383f072ece216,
title = "Biomolecular and electrochemical charge detection by a micromechanical electrometer",
abstract = "A micromechanical electrometer is applied to the label-free detection of biomolecular interactions and electrochemical charge sensing. The primary element of the electrometer is a micromechanical variable capacitor to modulate and convert a dc charge to an ac voltage output, thereby limiting the effects of low frequency noise on charge detection. At room temperature and ambient pressure the noise-limited charge resolution of a micromechanical electrometer based on this principle is found to be 3 e/ sqrt(Hz), enabling the potential detection of charged single molecule binding on electrode surfaces. The detection principle is validated by several experiments. Biomolecular binding experiments are conducted on an external gold electrode situated within a custom-designed flow cell and electrically connected to the micromachined electrometer. The concepts are validated by demonstrating the detection of biotin-streptavidin binding and DNA hybridization. Furthermore, it is shown that the electrometer can be applied for the detection of the redox system ferrocyanide/ferricyanide to describe Nernstian behaviour due to well defined charge transfer on the electrode surface at different concentration ratios as expected.",
author = "Andreas Menzel and Lin, {Angel T -H} and Pedro Estrela and Peng Li and Seshia, {Ashwin A}",
year = "2011",
month = "12",
day = "15",
doi = "10.1016/j.snb.2011.07.051",
language = "English",
volume = "160",
pages = "301--305",
journal = "Sensors and Actuators B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Biomolecular and electrochemical charge detection by a micromechanical electrometer

AU - Menzel, Andreas

AU - Lin, Angel T -H

AU - Estrela, Pedro

AU - Li, Peng

AU - Seshia, Ashwin A

PY - 2011/12/15

Y1 - 2011/12/15

N2 - A micromechanical electrometer is applied to the label-free detection of biomolecular interactions and electrochemical charge sensing. The primary element of the electrometer is a micromechanical variable capacitor to modulate and convert a dc charge to an ac voltage output, thereby limiting the effects of low frequency noise on charge detection. At room temperature and ambient pressure the noise-limited charge resolution of a micromechanical electrometer based on this principle is found to be 3 e/ sqrt(Hz), enabling the potential detection of charged single molecule binding on electrode surfaces. The detection principle is validated by several experiments. Biomolecular binding experiments are conducted on an external gold electrode situated within a custom-designed flow cell and electrically connected to the micromachined electrometer. The concepts are validated by demonstrating the detection of biotin-streptavidin binding and DNA hybridization. Furthermore, it is shown that the electrometer can be applied for the detection of the redox system ferrocyanide/ferricyanide to describe Nernstian behaviour due to well defined charge transfer on the electrode surface at different concentration ratios as expected.

AB - A micromechanical electrometer is applied to the label-free detection of biomolecular interactions and electrochemical charge sensing. The primary element of the electrometer is a micromechanical variable capacitor to modulate and convert a dc charge to an ac voltage output, thereby limiting the effects of low frequency noise on charge detection. At room temperature and ambient pressure the noise-limited charge resolution of a micromechanical electrometer based on this principle is found to be 3 e/ sqrt(Hz), enabling the potential detection of charged single molecule binding on electrode surfaces. The detection principle is validated by several experiments. Biomolecular binding experiments are conducted on an external gold electrode situated within a custom-designed flow cell and electrically connected to the micromachined electrometer. The concepts are validated by demonstrating the detection of biotin-streptavidin binding and DNA hybridization. Furthermore, it is shown that the electrometer can be applied for the detection of the redox system ferrocyanide/ferricyanide to describe Nernstian behaviour due to well defined charge transfer on the electrode surface at different concentration ratios as expected.

UR - http://www.scopus.com/inward/record.url?scp=81155152242&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1016/j.snb.2011.07.051

U2 - 10.1016/j.snb.2011.07.051

DO - 10.1016/j.snb.2011.07.051

M3 - Article

VL - 160

SP - 301

EP - 305

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

IS - 1

ER -