Abstract
The development of portable and sensitive biosensors for the label-free detection of DNA has influenced fundamental biological research as well as advanced applications. Here, we report the novel microchip-based electronic devices for the efficient and sensitive detection of a crop virus i.e. Cotton leaf curl Khokran virus-Burewala strain (CLCuKoV-Bur), predominant cotton infecting virus worldwide. Three-dimensional copper nanostructures reinforced graphene nanohybrid (Cu Ns@GO) is developed via a biological synthesis approach. The hybrid consists of the typical graphene sheets, embellished with copper nanoparticles of 10-15 nm, to impart conducting and metallic character. This nanohybrid is applied as the active interface of microchips, and the surface charge of +38 mV enables successful anchoring of virus DNA as a probe to the interface. The hybridization events are manipulated as the change in the electron transport between sheets of graphene, leading to a corresponding decrease in conductance of the devices. Termed as Cu Ns@GO-microchip, the devices can detect the presence of viral DNA down to the detection limit of 200 pM. We further investigate specificity patterns and the non-complementary DNAs i.e. Cotton leaf curl Multan betasatellite, Cotton leaf curl Multan alphasatellite, and Maize insect resistance1-cystein protease genes show a negligible response generating only 10-20% of the signal. We apply this strategy to the virus-infected cotton field samples and using the devices, the level of virus infectivity can be discerned. This study suggests the potential of nanotechnology-based microchips for designing benchmark recognition interfaces, for the direct and facile monitoring of agricultural pathogens and other bio-threats.
Original language | English |
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Pages (from-to) | 727-738 |
Number of pages | 12 |
Journal | Journal of Materials Research and Technology |
Volume | 12 |
Early online date | 10 Mar 2021 |
DOIs | |
Publication status | Published - 31 May 2021 |
Bibliographical note
Funding Information:Dr Sadia Z. Bajwa and Dr Imran Amin greatly acknowledge the financial support of Higher Education Commission (HEC) of Pakistan via Grant Number NRPU 6117 and NRPU 6116 .
Funding
Dr Sadia Z. Bajwa and Dr Imran Amin greatly acknowledge the financial support of Higher Education Commission (HEC) of Pakistan via Grant Number NRPU 6117 and NRPU 6116 .
Keywords
- DNA
- Graphene oxide interface
- Microchip
- Nanohybrid
- Virus
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Surfaces, Coatings and Films
- Metals and Alloys