TY - JOUR
T1 - Binding of individual Bacillus thuringiensis Cry proteins to the olive moth Prays oleae (Lepidoptera Yponomeutidae)
AU - Hernández-Rodríguez, Carmen Sara
AU - Pérez-Guerrero, Sergio
AU - Aldebis, Hani K
AU - Vargas-Osuna, Enrique
AU - Ferré, Juan
PY - 2009
Y1 - 2009
N2 - The microlepidopteran Prays oleae is one of the main insect pests causing significant crop losses in the Mediterranean olive groves. Bacillus thuringiensis based insecticides are being successfully used to minimize the impact of the second and third generations of this pest. However, because of its very small size and difficulty of rearing, very few studies have been carried out to determine the potency and mode of action of B. thuringiensis Cry proteins in this insect. In this study, Cry1Ac, Cry1Ca, and Cry1Fa proteins were shown to be toxic to third instar larvae of P. oleae. Furthermore, binding assays with (125)I-Cry1Ac and brush border membrane vesicles from midguts of last-instar larvae showed specific binding sites for Cry1Ac that are shared, with low affinity, by Cry1Ca and Cry1Fa.
AB - The microlepidopteran Prays oleae is one of the main insect pests causing significant crop losses in the Mediterranean olive groves. Bacillus thuringiensis based insecticides are being successfully used to minimize the impact of the second and third generations of this pest. However, because of its very small size and difficulty of rearing, very few studies have been carried out to determine the potency and mode of action of B. thuringiensis Cry proteins in this insect. In this study, Cry1Ac, Cry1Ca, and Cry1Fa proteins were shown to be toxic to third instar larvae of P. oleae. Furthermore, binding assays with (125)I-Cry1Ac and brush border membrane vesicles from midguts of last-instar larvae showed specific binding sites for Cry1Ac that are shared, with low affinity, by Cry1Ca and Cry1Fa.
UR - http://www.scopus.com/inward/record.url?scp=58649115807&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.jip.2008.11.005
U2 - 10.1016/j.jip.2008.11.005
DO - 10.1016/j.jip.2008.11.005
M3 - Article
C2 - 19041324
VL - 100
SP - 131
EP - 133
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
IS - 2
ER -