Binding of individual Bacillus thuringiensis Cry proteins to the olive moth Prays oleae (Lepidoptera Yponomeutidae)

Carmen Sara Hernández-Rodríguez, Sergio Pérez-Guerrero, Hani K Aldebis, Enrique Vargas-Osuna, Juan Ferré

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The microlepidopteran Prays oleae is one of the main insect pests causing significant crop losses in the Mediterranean olive groves. Bacillus thuringiensis based insecticides are being successfully used to minimize the impact of the second and third generations of this pest. However, because of its very small size and difficulty of rearing, very few studies have been carried out to determine the potency and mode of action of B. thuringiensis Cry proteins in this insect. In this study, Cry1Ac, Cry1Ca, and Cry1Fa proteins were shown to be toxic to third instar larvae of P. oleae. Furthermore, binding assays with (125)I-Cry1Ac and brush border membrane vesicles from midguts of last-instar larvae showed specific binding sites for Cry1Ac that are shared, with low affinity, by Cry1Ca and Cry1Fa.
Original languageEnglish
Pages (from-to)131-133
Number of pages3
JournalJournal of Invertebrate Pathology
Volume100
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint Dive into the research topics of 'Binding of individual Bacillus thuringiensis Cry proteins to the olive moth Prays oleae (Lepidoptera Yponomeutidae)'. Together they form a unique fingerprint.

Cite this