Bacterial polysaccharides suppress induced innate immunity by calcium chelation

Shazia N Aslam, Mari-Anne Newman, Gitte Erbs, Kate L Morrissey, Delphine Chinchilla, Thomas Boller, Tina Tandrup Jensen, Cristina De Castro, Teresa Ierano, Antonio Molinaro, Robert W Jackson, Marc R Knight, Richard M Cooper

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Abstract

Bacterial pathogens and symbionts must suppress or negate host innate immunity. However, pathogens release conserved oligomeric and polymeric molecules or MAMPs (Microbial Associated Molecular Patterns), which elicit host defenses [1], [2] and [3]. Extracellular polysaccharides (EPSs) are key virulence factors in plant and animal pathogenesis, but their precise function in establishing basic compatibility remains unclear [4], [5], [6] and [7]. Here, we show that EPSs suppress MAMP-induced signaling in plants through their polyanionic nature [4] and consequent ability to chelate divalent calcium ions [8]. In plants, Ca2+ ion influx to the cytosol from the apoplast (where bacteria multiply [4], [5] and [9]) is a prerequisite for activation of myriad defenses by MAMPs [10]. We show that EPSs from diverse plant and animal pathogens and symbionts bind calcium. EPS-defective mutants or pure MAMPs, such as the flagellin peptide flg22, elicit calcium influx, expression of host defense genes, and downstream resistance. Furthermore, EPSs, produced by wild-type strains or purified, suppress induced responses but do not block flg22-receptor binding in Arabidopsis cells. EPS production was confirmed in planta, and the amounts in bacterial biofilms greatly exceed those required for binding of apoplastic calcium. These data reveal a novel, fundamental role for bacterial EPS in disease establishment, encouraging novel control strategies.
LanguageEnglish
Pages1078-1083
Number of pages6
JournalCurrent Biology
Volume18
Issue number14
DOIs
StatusPublished - 22 Jul 2008

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Bacterial Polysaccharides
chelation
Chelation
Innate Immunity
Polysaccharides
polysaccharides
Calcium
calcium
Pathogens
Animals
symbionts
Ions
Flagellin
ions
animal pathogens
flagellin
Biofilms
Virulence Factors
pathogens
Arabidopsis

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Aslam, S. N., Newman, M-A., Erbs, G., Morrissey, K. L., Chinchilla, D., Boller, T., ... Cooper, R. M. (2008). Bacterial polysaccharides suppress induced innate immunity by calcium chelation. DOI: 10.1016/j.cub.2008.06.061

Bacterial polysaccharides suppress induced innate immunity by calcium chelation. / Aslam, Shazia N; Newman, Mari-Anne; Erbs, Gitte; Morrissey, Kate L; Chinchilla, Delphine; Boller, Thomas; Jensen, Tina Tandrup; De Castro, Cristina; Ierano, Teresa; Molinaro, Antonio; Jackson, Robert W; Knight, Marc R; Cooper, Richard M.

In: Current Biology, Vol. 18, No. 14, 22.07.2008, p. 1078-1083.

Research output: Contribution to journalArticle

Aslam, SN, Newman, M-A, Erbs, G, Morrissey, KL, Chinchilla, D, Boller, T, Jensen, TT, De Castro, C, Ierano, T, Molinaro, A, Jackson, RW, Knight, MR & Cooper, RM 2008, 'Bacterial polysaccharides suppress induced innate immunity by calcium chelation' Current Biology, vol. 18, no. 14, pp. 1078-1083. DOI: 10.1016/j.cub.2008.06.061
Aslam SN, Newman M-A, Erbs G, Morrissey KL, Chinchilla D, Boller T et al. Bacterial polysaccharides suppress induced innate immunity by calcium chelation. Current Biology. 2008 Jul 22;18(14):1078-1083. Available from, DOI: 10.1016/j.cub.2008.06.061
Aslam, Shazia N ; Newman, Mari-Anne ; Erbs, Gitte ; Morrissey, Kate L ; Chinchilla, Delphine ; Boller, Thomas ; Jensen, Tina Tandrup ; De Castro, Cristina ; Ierano, Teresa ; Molinaro, Antonio ; Jackson, Robert W ; Knight, Marc R ; Cooper, Richard M. / Bacterial polysaccharides suppress induced innate immunity by calcium chelation. In: Current Biology. 2008 ; Vol. 18, No. 14. pp. 1078-1083
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