Bacteria-responsive intelligent wound dressing

Simultaneous In situ detection and inhibition of bacterial infection for accelerated wound healing

Jin Zhou, Danyu Yao, Zhiyong Qian, Sen Hou, Linhao Li, A. Tobias A. Jenkins, Yubo Fan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The evolved resistance of antibiotics exhibited by some dreaded clinical pathogens and formation of biofilms has caused life-threatening problems for patients with burns and other wounds. Here, in order to avoid antibiotic overuse, and thus decreasing the occurrence of antimicrobial-resistant bacteria, a theranostic wound dressing, composed of biocompatible UV-photocrosslinkable methacrylated gelatin (GelMA) encapsulating both antimicrobial and fluorescent vesicles, has been developed. The system can respond to the microbiological environment of the wound via a simple color change and antimicrobials release only when require and this is in essence passive as they do not respond to their local environments and benign bacteria, and only operates when pathogenic bacteria exist. Both in Vitro and in Vivo study demonstrated that the proposed wound dressing was able to kill/inhibit the growth of methicillin-resistant S. aureus and P. aeruginosa, whilst providing a visual warning of infection, due to vesicle bilayer membrane lysed by toxins secreted by the two strains of pathogens but not by a non-pathogenic Escherichia coli species. The strategy of microbiologically responsive wound dressing proposed here could also be used as a general methodology for the design and fabrication of bacterial responsive functional biomaterials that offer opportunities to combat drug-resistant bacterial infections.

Original languageEnglish
Pages (from-to)11-23
Number of pages13
JournalBiomaterials
Volume161
Early online date13 Jan 2018
DOIs
Publication statusPublished - 1 Apr 2018

Fingerprint

Bandages
Bacterial Infections
Wound Healing
Bacteria
Pathogens
Antibiotics
Wounds and Injuries
Anti-Bacterial Agents
Methicillin
Biofilms
Biocompatible Materials
Gelatin
Biomaterials
Escherichia coli
Methicillin Resistance
Color
Membranes
Fabrication
Microbial Drug Resistance
Burns

Keywords

  • Antibacterial
  • Bacterial infection
  • Microbiological response
  • Polydiacetylene vesicles
  • Wound dressing

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Bacteria-responsive intelligent wound dressing : Simultaneous In situ detection and inhibition of bacterial infection for accelerated wound healing. / Zhou, Jin; Yao, Danyu; Qian, Zhiyong; Hou, Sen; Li, Linhao; Jenkins, A. Tobias A.; Fan, Yubo.

In: Biomaterials, Vol. 161, 01.04.2018, p. 11-23.

Research output: Contribution to journalArticle

@article{ccdf2929a1644b408a34fd15b8b84c4a,
title = "Bacteria-responsive intelligent wound dressing: Simultaneous In situ detection and inhibition of bacterial infection for accelerated wound healing",
abstract = "The evolved resistance of antibiotics exhibited by some dreaded clinical pathogens and formation of biofilms has caused life-threatening problems for patients with burns and other wounds. Here, in order to avoid antibiotic overuse, and thus decreasing the occurrence of antimicrobial-resistant bacteria, a theranostic wound dressing, composed of biocompatible UV-photocrosslinkable methacrylated gelatin (GelMA) encapsulating both antimicrobial and fluorescent vesicles, has been developed. The system can respond to the microbiological environment of the wound via a simple color change and antimicrobials release only when require and this is in essence passive as they do not respond to their local environments and benign bacteria, and only operates when pathogenic bacteria exist. Both in Vitro and in Vivo study demonstrated that the proposed wound dressing was able to kill/inhibit the growth of methicillin-resistant S. aureus and P. aeruginosa, whilst providing a visual warning of infection, due to vesicle bilayer membrane lysed by toxins secreted by the two strains of pathogens but not by a non-pathogenic Escherichia coli species. The strategy of microbiologically responsive wound dressing proposed here could also be used as a general methodology for the design and fabrication of bacterial responsive functional biomaterials that offer opportunities to combat drug-resistant bacterial infections.",
keywords = "Antibacterial, Bacterial infection, Microbiological response, Polydiacetylene vesicles, Wound dressing",
author = "Jin Zhou and Danyu Yao and Zhiyong Qian and Sen Hou and Linhao Li and Jenkins, {A. Tobias A.} and Yubo Fan",
year = "2018",
month = "4",
day = "1",
doi = "10.1016/j.biomaterials.2018.01.024",
language = "English",
volume = "161",
pages = "11--23",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",

}

TY - JOUR

T1 - Bacteria-responsive intelligent wound dressing

T2 - Simultaneous In situ detection and inhibition of bacterial infection for accelerated wound healing

AU - Zhou, Jin

AU - Yao, Danyu

AU - Qian, Zhiyong

AU - Hou, Sen

AU - Li, Linhao

AU - Jenkins, A. Tobias A.

AU - Fan, Yubo

PY - 2018/4/1

Y1 - 2018/4/1

N2 - The evolved resistance of antibiotics exhibited by some dreaded clinical pathogens and formation of biofilms has caused life-threatening problems for patients with burns and other wounds. Here, in order to avoid antibiotic overuse, and thus decreasing the occurrence of antimicrobial-resistant bacteria, a theranostic wound dressing, composed of biocompatible UV-photocrosslinkable methacrylated gelatin (GelMA) encapsulating both antimicrobial and fluorescent vesicles, has been developed. The system can respond to the microbiological environment of the wound via a simple color change and antimicrobials release only when require and this is in essence passive as they do not respond to their local environments and benign bacteria, and only operates when pathogenic bacteria exist. Both in Vitro and in Vivo study demonstrated that the proposed wound dressing was able to kill/inhibit the growth of methicillin-resistant S. aureus and P. aeruginosa, whilst providing a visual warning of infection, due to vesicle bilayer membrane lysed by toxins secreted by the two strains of pathogens but not by a non-pathogenic Escherichia coli species. The strategy of microbiologically responsive wound dressing proposed here could also be used as a general methodology for the design and fabrication of bacterial responsive functional biomaterials that offer opportunities to combat drug-resistant bacterial infections.

AB - The evolved resistance of antibiotics exhibited by some dreaded clinical pathogens and formation of biofilms has caused life-threatening problems for patients with burns and other wounds. Here, in order to avoid antibiotic overuse, and thus decreasing the occurrence of antimicrobial-resistant bacteria, a theranostic wound dressing, composed of biocompatible UV-photocrosslinkable methacrylated gelatin (GelMA) encapsulating both antimicrobial and fluorescent vesicles, has been developed. The system can respond to the microbiological environment of the wound via a simple color change and antimicrobials release only when require and this is in essence passive as they do not respond to their local environments and benign bacteria, and only operates when pathogenic bacteria exist. Both in Vitro and in Vivo study demonstrated that the proposed wound dressing was able to kill/inhibit the growth of methicillin-resistant S. aureus and P. aeruginosa, whilst providing a visual warning of infection, due to vesicle bilayer membrane lysed by toxins secreted by the two strains of pathogens but not by a non-pathogenic Escherichia coli species. The strategy of microbiologically responsive wound dressing proposed here could also be used as a general methodology for the design and fabrication of bacterial responsive functional biomaterials that offer opportunities to combat drug-resistant bacterial infections.

KW - Antibacterial

KW - Bacterial infection

KW - Microbiological response

KW - Polydiacetylene vesicles

KW - Wound dressing

UR - http://www.scopus.com/inward/record.url?scp=85041431047&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2018.01.024

DO - 10.1016/j.biomaterials.2018.01.024

M3 - Article

VL - 161

SP - 11

EP - 23

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -