Bacillus subtilis can modulate its capacity and specificity for protein secretion through temporally controlled expression of the sipS gene for signal peptidase I

Albert Bolhuis, Alexei Sorokin, Vasco Azevedo, S. Dusko Ehrlich, Peter G. Braun, Anne De Jong, Gerard Venema, Sierd Bron, Jan Maarten Van Dijl

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Bacillus subtilis contains three chromosomally encoded type I signal peptidases (SipS, SipT and SipU), which remove signal peptides from secretory precursor proteins. In the present study the biological function of SipS and the regulation of its synthesis wsre analysed. Unlike the type I signal peptidase of Escherichia coli, SipS was essential neither for protein secretion nor viability of the cell. However, in the absence of SipS the rate of processing of several preproteins was reduced, and four of the seven major secreted proteins of B. subtilis were hardly detectable in this growth medium. Surprisingly, the processing of Bacillus amyloliquefaciens α-amylase and the secretion of at least two endogenous B. subtilis proteins was improved in the absence of SipS. These findings indicate that the substrate preference of SipS differs from that of SipT and SipU, and that SipS is an important factor determining the efficiency of protein secretion in B. subtilis. SipS is transcribed in a growth phase- and medium-dependent manner. In minimal medium, the growth phase-dependent transcription of sipS is controlled by the DegS-DegU two-component regulatory system, indicating that the expression of sipS is regulated by the same factors that control the expression of most genes for secreted degradative enzymes. Our observations suggest that B. subtilis can modulate its capacity and specificity for protein secretion through the controlled expression of sipS.

Original languageEnglish
Pages (from-to)605-618
Number of pages14
JournalMolecular Microbiology
Volume22
Issue number4
DOIs
Publication statusPublished - 30 Nov 1996

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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