Abstract

Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.

Original languageEnglish
Pages (from-to)19389-19396
Number of pages8
JournalJournal of the American Chemical Society
Volume141
Issue number49
Early online date27 Nov 2019
DOIs
Publication statusPublished - 11 Dec 2019

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

@article{341c0943de404684afa364eac797d4e2,
title = "Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy",
abstract = "Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.",
author = "Murfin, {Lloyd C.} and Maria Weber and Park, {Sang Jun} and Kim, {Won Tae} and Lopez-Alled, {Carlos M.} and McMullin, {Claire L.} and Fabienne Pradaux-Caggiano and Lyall, {Catherine L.} and Gabriele Kociok-K{\"o}hn and Jannis Wenk and Bull, {Steven D.} and Juyoung Yoon and Kim, {Hwan Myung} and James, {Tony D.} and Lewis, {Simon E.}",
year = "2019",
month = "12",
day = "11",
doi = "10.1021/jacs.9b09813",
language = "English",
volume = "141",
pages = "19389--19396",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "49",

}

TY - JOUR

T1 - Azulene-Derived Fluorescent Probe for Bioimaging

T2 - Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy

AU - Murfin, Lloyd C.

AU - Weber, Maria

AU - Park, Sang Jun

AU - Kim, Won Tae

AU - Lopez-Alled, Carlos M.

AU - McMullin, Claire L.

AU - Pradaux-Caggiano, Fabienne

AU - Lyall, Catherine L.

AU - Kociok-Köhn, Gabriele

AU - Wenk, Jannis

AU - Bull, Steven D.

AU - Yoon, Juyoung

AU - Kim, Hwan Myung

AU - James, Tony D.

AU - Lewis, Simon E.

PY - 2019/12/11

Y1 - 2019/12/11

N2 - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.

AB - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.

UR - http://www.scopus.com/inward/record.url?scp=85076384021&partnerID=8YFLogxK

U2 - 10.1021/jacs.9b09813

DO - 10.1021/jacs.9b09813

M3 - Article

C2 - 31773957

AN - SCOPUS:85076384021

VL - 141

SP - 19389

EP - 19396

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 49

ER -