Abstract
Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
| Original language | English |
|---|---|
| Pages (from-to) | 19389-19396 |
| Number of pages | 8 |
| Journal | Journal of the American Chemical Society |
| Volume | 141 |
| Issue number | 49 |
| Early online date | 27 Nov 2019 |
| DOIs | |
| Publication status | Published - 11 Dec 2019 |
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)
- Biochemistry
- Colloid and Surface Chemistry
Cite this
Azulene-Derived Fluorescent Probe for Bioimaging : Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy. / Murfin, Lloyd C.; Weber, Maria; Park, Sang Jun; Kim, Won Tae; Lopez-Alled, Carlos M.; McMullin, Claire L.; Pradaux-Caggiano, Fabienne; Lyall, Catherine L.; Kociok-Köhn, Gabriele; Wenk, Jannis; Bull, Steven D.; Yoon, Juyoung; Kim, Hwan Myung; James, Tony D.; Lewis, Simon E.
In: Journal of the American Chemical Society, Vol. 141, No. 49, 11.12.2019, p. 19389-19396.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Azulene-Derived Fluorescent Probe for Bioimaging
T2 - Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
AU - Murfin, Lloyd C.
AU - Weber, Maria
AU - Park, Sang Jun
AU - Kim, Won Tae
AU - Lopez-Alled, Carlos M.
AU - McMullin, Claire L.
AU - Pradaux-Caggiano, Fabienne
AU - Lyall, Catherine L.
AU - Kociok-Köhn, Gabriele
AU - Wenk, Jannis
AU - Bull, Steven D.
AU - Yoon, Juyoung
AU - Kim, Hwan Myung
AU - James, Tony D.
AU - Lewis, Simon E.
PY - 2019/12/11
Y1 - 2019/12/11
N2 - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
AB - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
UR - http://www.scopus.com/inward/record.url?scp=85076384021&partnerID=8YFLogxK
U2 - 10.1021/jacs.9b09813
DO - 10.1021/jacs.9b09813
M3 - Article
C2 - 31773957
AN - SCOPUS:85076384021
VL - 141
SP - 19389
EP - 19396
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 49
ER -