TY - JOUR
T1 - Azulene-Derived Fluorescent Probe for Bioimaging
T2 - Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
AU - Murfin, Lloyd C.
AU - Weber, Maria
AU - Park, Sang Jun
AU - Kim, Won Tae
AU - Lopez-Alled, Carlos M.
AU - McMullin, Claire L.
AU - Pradaux-Caggiano, Fabienne
AU - Lyall, Catherine L.
AU - Kociok-Köhn, Gabriele
AU - Wenk, Jannis
AU - Bull, Steven D.
AU - Yoon, Juyoung
AU - Kim, Hwan Myung
AU - James, Tony D.
AU - Lewis, Simon E.
PY - 2019/12/11
Y1 - 2019/12/11
N2 - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 665992
AB - Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 665992
UR - http://www.scopus.com/inward/record.url?scp=85076384021&partnerID=8YFLogxK
U2 - 10.1021/jacs.9b09813
DO - 10.1021/jacs.9b09813
M3 - Article
C2 - 31773957
AN - SCOPUS:85076384021
VL - 141
SP - 19389
EP - 19396
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 49
ER -
