Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TNL-encoding neighbour

Fang Xu, Chipan Zhu, Volkan Cevik, Kaeli Johnson, Yanan Liu, Kee Sohn, Jonathan D Jones, Eric B Holub, Xin Li

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C-terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high defence marker gene expression, and salicylic acid accumulation. To search for novel regulators involved in CHS3-mediated immune signaling, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. These mutants suggest that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located genomically adjacent to CHS3 was found to be fully required for chs3-2D-mediated autoimmunity. CSA1 is located 3.9 kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.

LanguageEnglish
Article number8792
JournalScientific Reports
Volume5
DOIs
StatusPublished - 5 Mar 2015

Fingerprint

Autoimmunity
Interleukin-1 Receptors
Alleles
Plant Immunity
Dwarfism
Proteins
Salicylic Acid
Innate Immunity
Nucleotides
Gene Expression
NLR Proteins

Keywords

  • Autoimmunity
  • Gene Expression Regulation, Plant
  • Genetic Complementation Test
  • Genetic Loci
  • Mutation
  • Phenotype
  • Plant Proteins
  • Plants
  • Recombination, Genetic
  • Signal Transduction
  • Journal Article

Cite this

Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TNL-encoding neighbour. / Xu, Fang; Zhu, Chipan; Cevik, Volkan; Johnson, Kaeli; Liu, Yanan; Sohn, Kee; Jones, Jonathan D; Holub, Eric B; Li, Xin.

In: Scientific Reports, Vol. 5, 8792, 05.03.2015.

Research output: Contribution to journalArticle

Xu, F, Zhu, C, Cevik, V, Johnson, K, Liu, Y, Sohn, K, Jones, JD, Holub, EB & Li, X 2015, 'Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TNL-encoding neighbour', Scientific Reports, vol. 5, 8792. https://doi.org/10.1038/srep08792
Xu, Fang ; Zhu, Chipan ; Cevik, Volkan ; Johnson, Kaeli ; Liu, Yanan ; Sohn, Kee ; Jones, Jonathan D ; Holub, Eric B ; Li, Xin. / Autoimmunity conferred by chs3-2D relies on CSA1, its adjacent TNL-encoding neighbour. In: Scientific Reports. 2015 ; Vol. 5.
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abstract = "Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C-terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high defence marker gene expression, and salicylic acid accumulation. To search for novel regulators involved in CHS3-mediated immune signaling, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. These mutants suggest that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located genomically adjacent to CHS3 was found to be fully required for chs3-2D-mediated autoimmunity. CSA1 is located 3.9 kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.",
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AU - Liu, Yanan

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AU - Jones, Jonathan D

AU - Holub, Eric B

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AB - Plant innate immunity depends on the function of a large number of intracellular immune receptor proteins, the majority of which are structurally similar to mammalian nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) proteins. CHILLING SENSITIVE 3 (CHS3) encodes an atypical Toll/Interleukin 1 Receptor (TIR)-type NLR protein with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C-terminus. The gain-of-function mutant allele chs3-2D exhibits severe dwarfism and constitutively activated defense responses, including enhanced resistance to virulent pathogens, high defence marker gene expression, and salicylic acid accumulation. To search for novel regulators involved in CHS3-mediated immune signaling, we conducted suppressor screens in the chs3-2D and chs3-2D pad4-1 genetic backgrounds. Alleles of sag101 and eds1-90 were isolated as complete suppressors of chs3-2D, and alleles of sgt1b were isolated as partial suppressors of chs3-2D pad4-1. These mutants suggest that SAG101, EDS1-90, and SGT1b are all positive regulators of CHS3-mediated defense signaling. Additionally, the TIR-type NLR-encoding CSA1 locus located genomically adjacent to CHS3 was found to be fully required for chs3-2D-mediated autoimmunity. CSA1 is located 3.9 kb upstream of CHS3 and is transcribed in the opposite direction. Altogether, these data illustrate the distinct genetic requirements for CHS3-mediated defense signaling.

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KW - Gene Expression Regulation, Plant

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KW - Genetic Loci

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KW - Phenotype

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KW - Signal Transduction

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