Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice

MB Hogarth, PJ Norsworthy, PJ Allen, PKE Trinder, M Loos, Bernard J Morley, MJ Walport, KA Davies

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Abstract

We have developed an ELISA to measure murine autoantibodies to the collagenous region (CLR) of Clq, using the whole human Clq molecule as the solid-phase ligand, in the presence of 1 M NaCl. The assay was validated by testing positive sera from 20 mice using purified mouse Clq, and from 10 mice using purified human Clq-CLR, as the solid-phase ligands. There were highly significant correlations between results obtained with human Clq(whole molecule) and: (i) mouse Clq (r(Sp) = 0.73, P < 0.001), and (ii) human Clq-CLR alone (r(Sp) = 0.86, P = 0.001). Antibodies to Clq were measured in 53 MRL/lpr, 17 BXSB and 25 NZB/W lupus-prone mice. Median (range) anti-Clq (CLR) antibody levels in MRL/lpr, BXSB, and NZB/W autoimmune mice aged 3 months were 22 (16-66), 21 (17-39) and 19 (15-27) EU, respectively. The median anti-Clq antibody level in MRL/lpr mice aged 5 months was 76 (35-142) EU, significantly higher than that at 3 months (U = 558, P < 0.0005). Median anti-Clq antibody level in NZB/W mice at 8 months was 37 (13-74) EU and in BXSB mice at 11 months was 62 (31-231) EU, significantly higher than corresponding values at 3 months (U = 326, and U = 4, P < 0.001, respectively). This is the first demonstration of anti-Clq (CLR) antibodies in NZB/W and BXSB mice. The pathologic significance and the potential utility of these antibodies for monitoring disease in lupus-prone mice are under evaluation.
Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalClinical And Experimental Immunology
Volume104
Issue number2
DOIs
Publication statusPublished - May 1996

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Autoantibodies
Antibodies
Anti-Idiotypic Antibodies
Inbred MRL lpr Mouse
Inbred NZB Mouse
Ligands
Enzyme-Linked Immunosorbent Assay
Serum

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Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice. / Hogarth, MB; Norsworthy, PJ; Allen, PJ; Trinder, PKE; Loos, M; Morley, Bernard J; Walport, MJ; Davies, KA.

In: Clinical And Experimental Immunology, Vol. 104, No. 2, 05.1996, p. 241-246.

Research output: Contribution to journalArticle

Hogarth, MB, Norsworthy, PJ, Allen, PJ, Trinder, PKE, Loos, M, Morley, BJ, Walport, MJ & Davies, KA 1996, 'Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice', Clinical And Experimental Immunology, vol. 104, no. 2, pp. 241-246. https://doi.org/10.1046/j.1365-2249.1996.19725.x
Hogarth, MB ; Norsworthy, PJ ; Allen, PJ ; Trinder, PKE ; Loos, M ; Morley, Bernard J ; Walport, MJ ; Davies, KA. / Autoantibodies to the collagenous region of C1q occur in three strains of lupus-prone mice. In: Clinical And Experimental Immunology. 1996 ; Vol. 104, No. 2. pp. 241-246.
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AU - Davies, KA

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AB - We have developed an ELISA to measure murine autoantibodies to the collagenous region (CLR) of Clq, using the whole human Clq molecule as the solid-phase ligand, in the presence of 1 M NaCl. The assay was validated by testing positive sera from 20 mice using purified mouse Clq, and from 10 mice using purified human Clq-CLR, as the solid-phase ligands. There were highly significant correlations between results obtained with human Clq(whole molecule) and: (i) mouse Clq (r(Sp) = 0.73, P < 0.001), and (ii) human Clq-CLR alone (r(Sp) = 0.86, P = 0.001). Antibodies to Clq were measured in 53 MRL/lpr, 17 BXSB and 25 NZB/W lupus-prone mice. Median (range) anti-Clq (CLR) antibody levels in MRL/lpr, BXSB, and NZB/W autoimmune mice aged 3 months were 22 (16-66), 21 (17-39) and 19 (15-27) EU, respectively. The median anti-Clq antibody level in MRL/lpr mice aged 5 months was 76 (35-142) EU, significantly higher than that at 3 months (U = 558, P < 0.0005). Median anti-Clq antibody level in NZB/W mice at 8 months was 37 (13-74) EU and in BXSB mice at 11 months was 62 (31-231) EU, significantly higher than corresponding values at 3 months (U = 326, and U = 4, P < 0.001, respectively). This is the first demonstration of anti-Clq (CLR) antibodies in NZB/W and BXSB mice. The pathologic significance and the potential utility of these antibodies for monitoring disease in lupus-prone mice are under evaluation.

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