Autoantibodies to mitochondrial and centromere antigens in primary biliary cirrhosis and systemic sclerosis

N J McHugh, I E James, K Fairburn, P J Maddison

Research output: Contribution to journalArticle

Abstract

High levels of IgG antibodies to the multi-enzyme complex bovine pyruvate dehydrogenase (PDHC) were detected by a sensitive ELISA in all patients with histologically proven primary biliary cirrhosis (PBC), in a minority of patients with systemic sclerosis without overt PBC or with chronic liver diseases of another cause and in no rheumatoid arthritis or normal controls. Most (15/21) but not all of these positive patients recognized a 70-kD (E2) component of PDHC on immunoblotting. Additional recognition of a centromere-associated 140-kD polypeptide was associated with the presence of scleroderma-like features (Raynaud's phenomenon and sclerodactyly) in PBC, further emphasizing the serological and clinical overlap between these two conditions.
Original languageEnglish
Pages (from-to)244-9
Number of pages6
JournalClinical And Experimental Immunology
Volume81
Issue number2
Publication statusPublished - 1990

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Biliary Liver Cirrhosis
Centromere
Systemic Scleroderma
Autoantibodies
Antigens
Pyruvate Dehydrogenase Complex
Raynaud Disease
Immunoblotting
Liver Diseases
Rheumatoid Arthritis
Chronic Disease
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Peptides
Antibodies
Enzymes

Cite this

Autoantibodies to mitochondrial and centromere antigens in primary biliary cirrhosis and systemic sclerosis. / McHugh, N J; James, I E; Fairburn, K; Maddison, P J.

In: Clinical And Experimental Immunology, Vol. 81, No. 2, 1990, p. 244-9.

Research output: Contribution to journalArticle

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AB - High levels of IgG antibodies to the multi-enzyme complex bovine pyruvate dehydrogenase (PDHC) were detected by a sensitive ELISA in all patients with histologically proven primary biliary cirrhosis (PBC), in a minority of patients with systemic sclerosis without overt PBC or with chronic liver diseases of another cause and in no rheumatoid arthritis or normal controls. Most (15/21) but not all of these positive patients recognized a 70-kD (E2) component of PDHC on immunoblotting. Additional recognition of a centromere-associated 140-kD polypeptide was associated with the presence of scleroderma-like features (Raynaud's phenomenon and sclerodactyly) in PBC, further emphasizing the serological and clinical overlap between these two conditions.

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