Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli

Bethany Lee Patenall, Hollie Hathaway, Maisem Laabei, Amber Young, Naing T. Thet, A. Toby A. Jenkins, Robert D Short, Sarah L. Allinson

Research output: Contribution to journalArticlepeer-review

Abstract

The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV-radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on Escherichia coli were assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test, respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, which did not resemble that for UV implying that CAP-produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences.
Original languageEnglish
Article numbergeab030
Pages (from-to)380-387
Number of pages8
JournalMutagenesis
Volume36
Issue number5
Early online date30 Aug 2021
DOIs
Publication statusPublished - 30 Sep 2021

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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