Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase

Taísa Magnani Dinamarco; Neil Andrew Brown; Ricardo Sergio Couto de Almeida; Patrícia Alves de Castro; Marcela Savoldi; Maria Helena de Souza Goldman; Gustavo Henrique Goldman

doi:10.1016/j.micinf.2012.05.003

Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase

Taísa Magnani Dinamarco
, Neil Andrew Brown
, Ricardo Sergio Couto de Almeida
, Patrícia Alves de Castro
, Marcela Savoldi
, Maria Helena de Souza Goldman
, Gustavo Henrique Goldman

Department of Life Sciences

University of São Paulo
State University of Londrina
Centro Nacional de Pesquisa em Energia e Materiais

Research output: Contribution to journal › Article › peer-review

16 Citations (SciVal)

Abstract

The Ca²⁺-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH^V83F, was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH^V83F mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH.

Original language	English
Pages (from-to)	922-929
Number of pages	8
Journal	Microbes and Infection
Volume	14
Issue number	11
DOIs	https://doi.org/10.1016/j.micinf.2012.05.003
Publication status	Published - 1 Sept 2012

Funding

This research was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) , Brazil. We would like to thank the anonymous reviewers for their comments and suggestions, Dr. Andréa Carla Quiapim and the LBMP- FFCLRP/USP for DNA sequencing, and Dr. Reinhard Fischer for sending the BiFC plasmids. Appendix A

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Aspergillus fumigatus
Calcineurin
Galleria mellonella
Nucleoside diphosphate kinase

ASJC Scopus subject areas

Microbiology
Immunology
Infectious Diseases

Access to Document

10.1016/j.micinf.2012.05.003

Cite this

@article{a65542faee364d1d9c21fb7663f00b32,

title = "Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase",

abstract = "The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoHV83F, was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoHV83F mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH.",

keywords = "Aspergillus fumigatus, Calcineurin, Galleria mellonella, Nucleoside diphosphate kinase",

author = "\{Magnani Dinamarco\}, Ta{\'i}sa and Brown, \{Neil Andrew\} and \{Couto de Almeida\}, \{Ricardo Sergio\} and \{Alves de Castro\}, Patr{\'i}cia and Marcela Savoldi and \{de Souza Goldman\}, \{Maria Helena\} and Goldman, \{Gustavo Henrique\}",

year = "2012",

month = sep,

day = "1",

doi = "10.1016/j.micinf.2012.05.003",

language = "English",

volume = "14",

pages = "922--929",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier Masson s.r.l.",

number = "11",

}

TY - JOUR

T1 - Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase

AU - Magnani Dinamarco, Taísa

AU - Brown, Neil Andrew

AU - Couto de Almeida, Ricardo Sergio

AU - Alves de Castro, Patrícia

AU - Savoldi, Marcela

AU - de Souza Goldman, Maria Helena

AU - Goldman, Gustavo Henrique

PY - 2012/9/1

Y1 - 2012/9/1

N2 - The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoHV83F, was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoHV83F mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH.

AB - The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoHV83F, was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoHV83F mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH.

KW - Aspergillus fumigatus

KW - Calcineurin

KW - Galleria mellonella

KW - Nucleoside diphosphate kinase

UR - https://www.scopus.com/pages/publications/84865570807

U2 - 10.1016/j.micinf.2012.05.003

DO - 10.1016/j.micinf.2012.05.003

M3 - Article

C2 - 22634424

AN - SCOPUS:84865570807

SN - 1286-4579

VL - 14

SP - 922

EP - 929

JO - Microbes and Infection

JF - Microbes and Infection

IS - 11

ER -

Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase

Abstract

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this