Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase

Taísa Magnani Dinamarco, Neil Andrew Brown, Ricardo Sergio Couto de Almeida, Patrícia Alves de Castro, Marcela Savoldi, Maria Helena de Souza Goldman, Gustavo Henrique Goldman

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoHV83F, was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoHV83F mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH.

Original languageEnglish
Pages (from-to)922-929
Number of pages8
JournalMicrobes and Infection
Volume14
Issue number11
DOIs
Publication statusPublished - 1 Sep 2012

Keywords

  • Aspergillus fumigatus
  • Calcineurin
  • Galleria mellonella
  • Nucleoside diphosphate kinase

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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