Asparaginyl endopeptidases: enzymology, applications and limitations

Research output: Contribution to journalArticlepeer-review

Abstract

Asparaginyl endopeptidases (AEP) are cysteine proteases found in mammalian and plant cells. Several AEP isoforms from plant species were found to exhibit transpeptidase activity which is integral for the key head-to-tail cyclisation reaction during the biosynthesis of cyclotides. Since many plant AEPs exhibit excellent enzyme kinetics for peptide ligation via a relatively short substrate recognition sequence, they have become appealing tools for peptide and protein modification. In this review, research focused on the enzymology of AEPs and their applications in polypeptide cyclisation and labelling will be presented. Importantly, the limitations of using AEPs and opportunities for future research and innovation will also be discussed.
Original languageEnglish
JournalOrganic & Biomolecular Chemistry
Issue number23
DOIs
Publication statusPublished - 12 May 2021

Fingerprint

Dive into the research topics of 'Asparaginyl endopeptidases: enzymology, applications and limitations'. Together they form a unique fingerprint.

Cite this