Abstract
We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.
| Original language | English |
|---|---|
| Article number | 093701 |
| Journal | Applied Physics Letters |
| Volume | 95 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sept 2009 |
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)