Arbitrary multisite two-photon excitation in four dimensions

Vincent Ricardo Daria, Christian Stricker, Richard Bowman, Stephen Redman, Hans A. Bachor

Research output: Contribution to journalArticlepeer-review

45 Citations (SciVal)


We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.

Original languageEnglish
Article number093701
JournalApplied Physics Letters
Issue number9
Publication statusPublished - Sept 2009

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)


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