Abstract
We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.
Original language | English |
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Article number | 093701 |
Journal | Applied Physics Letters |
Volume | 95 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sept 2009 |
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)