Aptamer-based Field-Effect Biosensor for Tenofovir Detection

Nima Aliakbarinodehi, Pawan Jolly, Nikhil Bhalla, Anna Miodek, Giovanni de Micheli, Pedro Estrela, Sandro Carrara

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer conditions, shows optimum specific and little non-specific binding in phosphate buffered saline. The dose-response behavior of the field-effect biosensor presents a linear range between 1 nM and 100 nM of tenofovir and a limit of detection of 1.2 nM. Two non-specific drugs and one non-specific aptamer, tested as stringent control candidates, caused negligible responses. The applications were successfully extended to the detection of the drug in human serum. As demonstrated by impedance measurements, the aptamer-based sensors can be used for real-time drug monitoring.
LanguageEnglish
Article number44409
Pages1-10
Number of pages10
JournalScientific Reports
Volume7
Early online date15 Mar 2017
DOIs
StatusPublished - 15 Mar 2017

Fingerprint

Tenofovir
Biosensors
Pharmaceutical Preparations
Monitoring
Sensors
Surface plasmon resonance
Self assembled monolayers
Field effect transistors
Electrochemical impedance spectroscopy
Transducers
Buffers
Phosphates

Keywords

  • Biosensors

Cite this

Aliakbarinodehi, N., Jolly, P., Bhalla, N., Miodek, A., de Micheli, G., Estrela, P., & Carrara, S. (2017). Aptamer-based Field-Effect Biosensor for Tenofovir Detection. Scientific Reports, 7, 1-10. [44409]. https://doi.org/10.1038/srep44409

Aptamer-based Field-Effect Biosensor for Tenofovir Detection. / Aliakbarinodehi, Nima; Jolly, Pawan; Bhalla, Nikhil; Miodek, Anna; de Micheli, Giovanni; Estrela, Pedro; Carrara, Sandro.

In: Scientific Reports, Vol. 7, 44409, 15.03.2017, p. 1-10.

Research output: Contribution to journalArticle

Aliakbarinodehi, N, Jolly, P, Bhalla, N, Miodek, A, de Micheli, G, Estrela, P & Carrara, S 2017, 'Aptamer-based Field-Effect Biosensor for Tenofovir Detection', Scientific Reports, vol. 7, 44409, pp. 1-10. https://doi.org/10.1038/srep44409
Aliakbarinodehi N, Jolly P, Bhalla N, Miodek A, de Micheli G, Estrela P et al. Aptamer-based Field-Effect Biosensor for Tenofovir Detection. Scientific Reports. 2017 Mar 15;7:1-10. 44409. https://doi.org/10.1038/srep44409
Aliakbarinodehi, Nima ; Jolly, Pawan ; Bhalla, Nikhil ; Miodek, Anna ; de Micheli, Giovanni ; Estrela, Pedro ; Carrara, Sandro. / Aptamer-based Field-Effect Biosensor for Tenofovir Detection. In: Scientific Reports. 2017 ; Vol. 7. pp. 1-10.
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