Application of sonic hedgehog to the developing chick limb

Tiecke Eva , Cheryll Tickle

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Here, we describe methods for applying Sonic hedgehog (Shh) to developing chick limbs. The Sonic hedgehog gene is expressed in the polarizing region, a signaling region at the posterior margin of the limb bud and application of Shh-expressing cells or Shh protein to early limb buds mimics polarizing region signaling. The polarizing region (or zone of polarizing activity) is involved in one of the best known cell-cell interactions in vertebrate embryos and is pivotal in controlling digit number and pattern. At later stages of limb development, the application of Shh protein to the regions between digit primordia can induce changes in digit morphogenesis.
Original languageEnglish
Pages (from-to)23-33
JournalMethods in Molecular Biology
Volume397
DOIs
Publication statusPublished - 2007

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  • Projects

    Signalling in Vertebrate Limb Development

    Tickle, C. & Downie, H.

    Medical Research Council

    1/09/0731/12/08

    Project: Research council

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