Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Jeremy Bartosiak-Jentys; Kirstin Eley; David J Leak

doi:10.1128/AEM.07944-11

Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Jeremy Bartosiak-Jentys
, Kirstin Eley
, David J Leak

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

18 Citations (SciVal)

Abstract

The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.

Original language	English
Pages (from-to)	5945-5947
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	16
Early online date	8 Jun 2012
DOIs	https://doi.org/10.1128/AEM.07944-11
Publication status	Published - Aug 2012

Access to Document

10.1128/AEM.07944-11

Cite this

@article{e90f9f39358a40db84892cf1f799660f,

title = "Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength",

abstract = "The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.",

author = "Jeremy Bartosiak-Jentys and Kirstin Eley and Leak, \{David J\}",

year = "2012",

month = aug,

doi = "10.1128/AEM.07944-11",

language = "English",

volume = "78",

pages = "5945--5947",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "16",

}

TY - JOUR

T1 - Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

AU - Bartosiak-Jentys, Jeremy

AU - Eley, Kirstin

AU - Leak, David J

PY - 2012/8

Y1 - 2012/8

N2 - The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.

AB - The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.

UR - https://www.scopus.com/pages/publications/84866170627

UR - http://dx.doi.org/10.1128/AEM.07944-11

U2 - 10.1128/AEM.07944-11

DO - 10.1128/AEM.07944-11

M3 - Article

C2 - 22685159

SN - 0099-2240

VL - 78

SP - 5945

EP - 5947

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 16

ER -

Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Abstract

Access to Document

Other files and links

Fingerprint

Evaluation of Consolidated Bioprocessing as a Strategy for Production of Fuels and Chemicals from Lignocellulose

Cite this

Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Abstract

Access to Document

Other files and links

Fingerprint

Projects

Evaluation of Consolidated Bioprocessing as a Strategy for Production of Fuels and Chemicals from Lignocellulose

Cite this