Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Jeremy Bartosiak-Jentys, Kirstin Eley, David J Leak

Research output: Contribution to journalArticle

12 Citations (Scopus)


The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
Original languageEnglish
Pages (from-to)5945-5947
Number of pages3
JournalApplied and Environmental Microbiology
Issue number16
Early online date8 Jun 2012
Publication statusPublished - Aug 2012


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