TY - JOUR
T1 - Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength
AU - Bartosiak-Jentys, Jeremy
AU - Eley, Kirstin
AU - Leak, David J
PY - 2012/8
Y1 - 2012/8
N2 - The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
AB - The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
UR - http://www.scopus.com/inward/record.url?scp=84866170627&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1128/AEM.07944-11
U2 - 10.1128/AEM.07944-11
DO - 10.1128/AEM.07944-11
M3 - Article
C2 - 22685159
VL - 78
SP - 5945
EP - 5947
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 16
ER -