Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Jeremy Bartosiak-Jentys; Kirstin Eley; David J Leak

doi:10.1128/AEM.07944-11

Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Jeremy Bartosiak-Jentys, Kirstin Eley, David J Leak

Department of Biology & Biochemistry

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.

Original language	English
Pages (from-to)	5945-5947
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	16
Early online date	8 Jun 2012
DOIs	https://doi.org/10.1128/AEM.07944-11
Publication status	Published - Aug 2012

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abstract = "The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.",

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