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Abstract
The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
Original language | English |
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Pages (from-to) | 5945-5947 |
Number of pages | 3 |
Journal | Applied and Environmental Microbiology |
Volume | 78 |
Issue number | 16 |
Early online date | 8 Jun 2012 |
DOIs | |
Publication status | Published - Aug 2012 |
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Dive into the research topics of 'Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength'. Together they form a unique fingerprint.Projects
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Evaluation of Consolidated Bioprocessing as a Strategy for Production of Fuels and Chemicals from Lignocellulose
Leak, D. (PI)
Biotechnology and Biological Sciences Research Council
15/04/12 → 8/04/14
Project: Research council