Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength

Jeremy Bartosiak-Jentys, Kirstin Eley, David J Leak

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
Original languageEnglish
Pages (from-to)5945-5947
Number of pages3
JournalApplied and Environmental Microbiology
Volume78
Issue number16
Early online date8 Jun 2012
DOIs
Publication statusPublished - Aug 2012

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Geobacillus
Reporter Genes
reporter genes
quantitative analysis
promoter regions
screening
Geobacillus stearothermophilus
catechol
gene
Genes
genes
metabolism

Cite this

Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength. / Bartosiak-Jentys, Jeremy; Eley, Kirstin; Leak, David J.

In: Applied and Environmental Microbiology, Vol. 78, No. 16, 08.2012, p. 5945-5947.

Research output: Contribution to journalArticle

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