Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 5945-5947 |
| Number of pages | 3 |
| Journal | Applied and Environmental Microbiology |
| Volume | 78 |
| Issue number | 16 |
| Early online date | 8 Jun 2012 |
| DOIs | |
| Publication status | Published - Aug 2012 |
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Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength. / Bartosiak-Jentys, Jeremy; Eley, Kirstin; Leak, David J.
In: Applied and Environmental Microbiology, Vol. 78, No. 16, 08.2012, p. 5945-5947.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Application of pheB as a reporter gene for Geobacillus spp, enabling qualitative colony screening and quantitative analysis of promoter strength
AU - Bartosiak-Jentys, Jeremy
AU - Eley, Kirstin
AU - Leak, David J
PY - 2012/8
Y1 - 2012/8
N2 - The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
AB - The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3dioxygenase (C23O), catalyses the formation of 2-hydroxymuconic semialdehyde which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
UR - http://www.scopus.com/inward/record.url?scp=84866170627&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1128/AEM.07944-11
U2 - 10.1128/AEM.07944-11
DO - 10.1128/AEM.07944-11
M3 - Article
VL - 78
SP - 5945
EP - 5947
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 16
ER -