The electrochemically coated conducting paper based point-of-care diagnostics using amperometric sensing principles for troponin detection has been demonstrated in the present report. The cost-effective and stable conducting polymer coating was achieved on papers using screen printing followed by electrochemical deposition techniques for the first time. The cyclic voltammetric analysis has revealed the stable and uniform conducting polyaniline (PANI) coating onto filter papers fabricated using the simple and novel approach. These strips were found to be very sensitive for the futuristic diagnostics and the concept was proved by utilizing these strips for the troponin detection in a wide physiological range (1–100 ng/mL) with sensitivity of 5.5 μA/ng mL− 1 cm− 2. Association constant of the electrode has been found to be 1.2 × 1013 L mol− 1.