Antiviral Decoction of Isatidis Radix ( bn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity

Lijing Ke, Teng Wen, Jeremy Bradshaw, Jianwu Zhou, Pingfan Rao

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The aim of this study is to elucidate how the Isatidis Radix ( bn lán gēn) tonic, as an aqueous mixture of hundreds of compositions, interrupts the infection of influenza viruses to their host cells. The efficacy of the tonic was evaluated and expressed as cell proliferation rate and plaque reduction rate in Madin-Darby Canine Kidney (MDCK) cells, against 3 strains of influenza A and B viruses. This boiling water (at 100°C ) extract of Isatidis Radix (RIE) showed antiviral activity against influenza virus A and B. The concentration for 50% inhibition of influenza virus A replication (IC50) in MDCK cell was 12.6 mg/mL with a therapeutic index >8. When cells were incubated with RIE prior to virus dsorption, the numbers of viable cell were at least doubled compared to the numbers of virus control, RIE incubation after virus adsorption and RIE incubation with virus prior to adsorption, in both influenza virus A and B. Moreover, much less virus particles were spotted by scanning electron microscope (SEM) in the RIE pre-treated cells than the cells without RIE treatment. These results indicate the antiviral activity of RIE is mainly attributed to its host cell protection effect but not actions on virus or post-virus-adsorption interruption. Cell, but not virus, is more likely to be the action target of RIE.

Original languageEnglish
Pages (from-to)47-51
Number of pages5
JournalJournal of Traditional and Complementary Medicine
Volume2
Issue number1
DOIs
Publication statusE-pub ahead of print - 16 Jul 2016

Keywords

  • Antiviral effects
  • Cell binding
  • Cell protection
  • Influenza virus
  • Isatidis Radix ( bn lán gēn)

ASJC Scopus subject areas

  • Complementary and alternative medicine

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