Abstract
The aim of this study is to elucidate how the Isatidis Radix ( bn lán gēn) tonic, as an aqueous mixture of hundreds of compositions, interrupts the infection of influenza viruses to their host cells. The efficacy of the tonic was evaluated and expressed as cell proliferation rate and plaque reduction rate in Madin-Darby Canine Kidney (MDCK) cells, against 3 strains of influenza A and B viruses. This boiling water (at 100°C ) extract of Isatidis Radix (RIE) showed antiviral activity against influenza virus A and B. The concentration for 50% inhibition of influenza virus A replication (IC50) in MDCK cell was 12.6 mg/mL with a therapeutic index >8. When cells were incubated with RIE prior to virus dsorption, the numbers of viable cell were at least doubled compared to the numbers of virus control, RIE incubation after virus adsorption and RIE incubation with virus prior to adsorption, in both influenza virus A and B. Moreover, much less virus particles were spotted by scanning electron microscope (SEM) in the RIE pre-treated cells than the cells without RIE treatment. These results indicate the antiviral activity of RIE is mainly attributed to its host cell protection effect but not actions on virus or post-virus-adsorption interruption. Cell, but not virus, is more likely to be the action target of RIE.
Original language | English |
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Pages (from-to) | 47-51 |
Number of pages | 5 |
Journal | Journal of Traditional and Complementary Medicine |
Volume | 2 |
Issue number | 1 |
DOIs | |
Publication status | E-pub ahead of print - 16 Jul 2016 |
Funding
This research was supported by the National Basic Research Program of China (Grant No.2010CB530605); National Natural Science Foundation of China (Grant No. No.30973871). Fujian Centre of Disease Control (Fuzhou, Fujian, P.R.China) is acknowledged for generous providing the influenza virus strains and part of virological study facilities. Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co. Ltd. (Guangzhou, P.R.China) is acknowledged for providing the raw Isatidis Radix materials.
Keywords
- Antiviral effects
- Cell binding
- Cell protection
- Influenza virus
- Isatidis Radix ( bn lán gēn)
ASJC Scopus subject areas
- Complementary and alternative medicine