'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte

Maria L. Odyniec, Adam C. Sedgwick, Alexander H. Swan, Maria Weber, T. M. Simon Tang, Jordan E. Gardiner, Miao Zhang, Yun Bao Jiang, Gabriele Kociok-Kohn, Robert B.P. Elmes, Steven D. Bull, Xiao Peng He, Tony D. James

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Traditionally, fluorescence probes have focused on the detection of a single biomarker for a specific process. In this work, we set out to develop a number of fluorescence probes that enable the detection of a chosen analyte in the presence of reactive oxygen/nitrogen species (ROS/RNS). These fluorescence probes when activated result in the formation of the highly fluorescent pink dye, resorufin. Therefore, we have labelled these fluorescent probes as -Pinkments'. Our first -Pinkment' was shown to detect biologically relevant concentrations of ONOO- and have an excellent selectivity against other ROS/RNS. Pinkment-OH was developed to provide a core unit which could be easily functionalised to produce a range of -AND' based fluorescence probes for the detection of ROS/RNS and a second analyte. For proof of concept, we synthesised Pinkment-OTBS and Pinkment-OAc. These -AND'-based probes were successfully shown to detect ROS/RNS and F- or esterase, respectively.

LanguageEnglish
Pages8466-8469
Number of pages4
JournalChemical Communications
Volume54
Early online date27 Jun 2018
DOIs
StatusE-pub ahead of print - 27 Jun 2018

Fingerprint

Scaffolds
Fluorescence
Biomarkers
Esterases
Fluorescent Dyes
Coloring Agents
Nitrogen
Oxygen
Dyes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte. / Odyniec, Maria L.; Sedgwick, Adam C.; Swan, Alexander H.; Weber, Maria; Tang, T. M. Simon; Gardiner, Jordan E.; Zhang, Miao; Jiang, Yun Bao; Kociok-Kohn, Gabriele; Elmes, Robert B.P.; Bull, Steven D.; He, Xiao Peng; James, Tony D.

In: Chemical Communications, Vol. 54, 27.06.2018, p. 8466-8469.

Research output: Contribution to journalArticle

Odyniec, Maria L. ; Sedgwick, Adam C. ; Swan, Alexander H. ; Weber, Maria ; Tang, T. M. Simon ; Gardiner, Jordan E. ; Zhang, Miao ; Jiang, Yun Bao ; Kociok-Kohn, Gabriele ; Elmes, Robert B.P. ; Bull, Steven D. ; He, Xiao Peng ; James, Tony D. / 'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte. In: Chemical Communications. 2018 ; Vol. 54. pp. 8466-8469.
@article{4d3d17068b7044b18e6398bea9ff1df0,
title = "'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte",
abstract = "Traditionally, fluorescence probes have focused on the detection of a single biomarker for a specific process. In this work, we set out to develop a number of fluorescence probes that enable the detection of a chosen analyte in the presence of reactive oxygen/nitrogen species (ROS/RNS). These fluorescence probes when activated result in the formation of the highly fluorescent pink dye, resorufin. Therefore, we have labelled these fluorescent probes as -Pinkments'. Our first -Pinkment' was shown to detect biologically relevant concentrations of ONOO- and have an excellent selectivity against other ROS/RNS. Pinkment-OH was developed to provide a core unit which could be easily functionalised to produce a range of -AND' based fluorescence probes for the detection of ROS/RNS and a second analyte. For proof of concept, we synthesised Pinkment-OTBS and Pinkment-OAc. These -AND'-based probes were successfully shown to detect ROS/RNS and F- or esterase, respectively.",
author = "Odyniec, {Maria L.} and Sedgwick, {Adam C.} and Swan, {Alexander H.} and Maria Weber and Tang, {T. M. Simon} and Gardiner, {Jordan E.} and Miao Zhang and Jiang, {Yun Bao} and Gabriele Kociok-Kohn and Elmes, {Robert B.P.} and Bull, {Steven D.} and He, {Xiao Peng} and James, {Tony D.}",
year = "2018",
month = "6",
day = "27",
doi = "10.1039/c8cc04316g",
language = "English",
volume = "54",
pages = "8466--8469",
journal = "Chemical communications (Cambridge, England)",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",

}

TY - JOUR

T1 - 'AND'-based fluorescence scaffold for the detection of ROS/RNS and a second analyte

AU - Odyniec, Maria L.

AU - Sedgwick, Adam C.

AU - Swan, Alexander H.

AU - Weber, Maria

AU - Tang, T. M. Simon

AU - Gardiner, Jordan E.

AU - Zhang, Miao

AU - Jiang, Yun Bao

AU - Kociok-Kohn, Gabriele

AU - Elmes, Robert B.P.

AU - Bull, Steven D.

AU - He, Xiao Peng

AU - James, Tony D.

PY - 2018/6/27

Y1 - 2018/6/27

N2 - Traditionally, fluorescence probes have focused on the detection of a single biomarker for a specific process. In this work, we set out to develop a number of fluorescence probes that enable the detection of a chosen analyte in the presence of reactive oxygen/nitrogen species (ROS/RNS). These fluorescence probes when activated result in the formation of the highly fluorescent pink dye, resorufin. Therefore, we have labelled these fluorescent probes as -Pinkments'. Our first -Pinkment' was shown to detect biologically relevant concentrations of ONOO- and have an excellent selectivity against other ROS/RNS. Pinkment-OH was developed to provide a core unit which could be easily functionalised to produce a range of -AND' based fluorescence probes for the detection of ROS/RNS and a second analyte. For proof of concept, we synthesised Pinkment-OTBS and Pinkment-OAc. These -AND'-based probes were successfully shown to detect ROS/RNS and F- or esterase, respectively.

AB - Traditionally, fluorescence probes have focused on the detection of a single biomarker for a specific process. In this work, we set out to develop a number of fluorescence probes that enable the detection of a chosen analyte in the presence of reactive oxygen/nitrogen species (ROS/RNS). These fluorescence probes when activated result in the formation of the highly fluorescent pink dye, resorufin. Therefore, we have labelled these fluorescent probes as -Pinkments'. Our first -Pinkment' was shown to detect biologically relevant concentrations of ONOO- and have an excellent selectivity against other ROS/RNS. Pinkment-OH was developed to provide a core unit which could be easily functionalised to produce a range of -AND' based fluorescence probes for the detection of ROS/RNS and a second analyte. For proof of concept, we synthesised Pinkment-OTBS and Pinkment-OAc. These -AND'-based probes were successfully shown to detect ROS/RNS and F- or esterase, respectively.

UR - http://www.scopus.com/inward/record.url?scp=85050767455&partnerID=8YFLogxK

U2 - 10.1039/c8cc04316g

DO - 10.1039/c8cc04316g

M3 - Article

VL - 54

SP - 8466

EP - 8469

JO - Chemical communications (Cambridge, England)

T2 - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

SN - 1359-7345

ER -