Anatomy of the bacitracin resistance network in Bacillus subtilis

Jara Radeck, Susanne Gebhard, Peter Shevlin Orchard, Marion Kirchner, Stephanie Bauer, Thorsten Mascher, Georg Fritz

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Abstract

Protection against antimicrobial peptides (AMPs) often involves the parallel production of multiple, well-characterized resistance determinants. So far, little is known about how these resistance modules interact and how they jointly protect the cell. Here, we studied the interdependence between different layers of the envelope stress response of Bacillus subtilis when challenged with the lipid II cycle-inhibiting AMP bacitracin. The underlying regulatory network orchestrates the production of the ABC transporter BceAB, the UPP phosphatase BcrCand the phage-shock proteins LiaIH. Our systems-level analysis reveals a clear hierarchy,allowing us to discriminate between primary (BceAB) and secondary (BcrC and LiaIH) layers ofbacitracin resistance. Deleting the primary layer provokes an enhanced induction of the secondary layer to partially compensate for this loss. This study reveals a direct role of LiaI H inbacitracin resistance, provides novel insights into the feedback regulation of the Lia system, anddemonstrates a pivotal role of BcrC in maintaining cell wall homeostasis. The compensatory regulation within the bacitracin network can also explain how gene expression noise propagates between resistance layers. We suggest that this active redundancy in the bacitracin resistance network of B. subtilis is a general principle to be found in many bacterial antibiotic resistance networks.
Original languageEnglish
Pages (from-to)607-620
JournalMolecular Microbiology
Volume100
Issue number4
Early online date27 Jan 2016
DOIs
Publication statusPublished - 10 Mar 2016

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Radeck, J., Gebhard, S., Orchard, P. S., Kirchner, M., Bauer, S., Mascher, T., & Fritz, G. (2016). Anatomy of the bacitracin resistance network in Bacillus subtilis. Molecular Microbiology, 100(4), 607-620. https://doi.org/10.1111/mmi.13336