Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis

Marta P Pereira Morais, John S Fossey, Tony D James, Johannes van den Elsen

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Carbohydrate modification of proteins adds complexity and diversity to the proteome. However, undesired carbohydrate modifications also occur in the form of glycation, resulting in diseases such as diabetes, Alzheimer's disease, autoimmune diseases, and cancer. The analysis of glycated proteins is challenging due to their complexity and variability. Numerous analytical techniques have been developed that require expensive specialised equipment and complex data analysis. In this chapter, we describe a simple electrophoresis-based method that enables users to detect, identify, and analyze these post-translational modifications. This new cost-effective methodology will aid the detection of unwanted glycation products in processed foods and may lead to new diagnostics and therapeutics for age-related chronic diseases and glycosylation disorders.
Original languageEnglish
Title of host publicationProtein Electrophoresis
EditorsBiji T Kurien, R Hall Scofield
PublisherSpringer
Pages93-109
Number of pages17
Edition869
ISBN (Print)978-1-61779-821-4_9
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherSpringer

Fingerprint

Acrylamide
Electrophoresis
Gels
Proteins
Processed foods
Carbohydrates
Glycosylation
Proteome
Medical problems
benzeneboronic acid
Costs

Cite this

Pereira Morais, M. P., Fossey, J. S., James, T. D., & van den Elsen, J. (2012). Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis. In B. T. Kurien, & R. H. Scofield (Eds.), Protein Electrophoresis (869 ed., pp. 93-109). (Methods in Molecular Biology). Springer. https://doi.org/10.1007/978-1-61779-821-4_9

Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis. / Pereira Morais, Marta P; Fossey, John S; James, Tony D; van den Elsen, Johannes.

Protein Electrophoresis. ed. / Biji T Kurien; R Hall Scofield. 869. ed. Springer, 2012. p. 93-109 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Pereira Morais, MP, Fossey, JS, James, TD & van den Elsen, J 2012, Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis. in BT Kurien & RH Scofield (eds), Protein Electrophoresis. 869 edn, Methods in Molecular Biology, Springer, pp. 93-109. https://doi.org/10.1007/978-1-61779-821-4_9
Pereira Morais MP, Fossey JS, James TD, van den Elsen J. Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis. In Kurien BT, Scofield RH, editors, Protein Electrophoresis. 869 ed. Springer. 2012. p. 93-109. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-821-4_9
Pereira Morais, Marta P ; Fossey, John S ; James, Tony D ; van den Elsen, Johannes. / Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis. Protein Electrophoresis. editor / Biji T Kurien ; R Hall Scofield. 869. ed. Springer, 2012. pp. 93-109 (Methods in Molecular Biology).
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