Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis

Marta P. Pereira Morais, Dominic Marshall, Stephen E. Flower, Christopher J. Caunt, Tony D. James, Robert J. Williams, Nicholas R. Waterfield, Jean M. H. Van Den Elsen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Glycated proteins are important biomarkers for age-related disorders, however their analysis is challenging because of the complexity of the protein-carbohydrate adducts. Here we report a method that enables the detection and identification of individual glycated proteins in complex samples using fluorescent boronic acids in gel electrophoresis. Using this method we identified glycated proteins in human serum, insect hemolymph and mouse brain homogenates, confirming this technique as a powerful proteomics tool that can be used for the identification of potential disease biomarkers.
Original languageEnglish
Article number1437
Pages (from-to)1-7
Number of pages7
JournalScientific Reports
Volume3
DOIs
Publication statusPublished - 27 Mar 2013

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