Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis

Marta P. Pereira Morais, Dominic Marshall, Stephen E. Flower, Christopher J. Caunt, Tony D. James, Robert J. Williams, Nicholas R. Waterfield, Jean M. H. Van Den Elsen

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Abstract

Glycated proteins are important biomarkers for age-related disorders, however their analysis is challenging because of the complexity of the protein-carbohydrate adducts. Here we report a method that enables the detection and identification of individual glycated proteins in complex samples using fluorescent boronic acids in gel electrophoresis. Using this method we identified glycated proteins in human serum, insect hemolymph and mouse brain homogenates, confirming this technique as a powerful proteomics tool that can be used for the identification of potential disease biomarkers.
LanguageEnglish
Article number1437
JournalScientific Reports
Volume3
DOIs
StatusPublished - 27 Mar 2013

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Electrophoresis
Gels
Biomarkers
Proteins
Boronic Acids
Brain
Carbohydrates
benzeneboronic acid

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Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis. / Pereira Morais, Marta P.; Marshall, Dominic; Flower, Stephen E.; Caunt, Christopher J.; James, Tony D.; Williams, Robert J.; Waterfield, Nicholas R.; Van Den Elsen, Jean M. H.

In: Scientific Reports, Vol. 3, 1437, 27.03.2013.

Research output: Contribution to journalArticle

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