Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis

Marta P. Pereira Morais; Dominic Marshall; Stephen E. Flower; Christopher J. Caunt; Tony D. James; Robert J. Williams; Nicholas R. Waterfield; Jean M. H. Van Den Elsen

doi:10.1038/srep01437

Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis

Marta P. Pereira Morais, Dominic Marshall, Stephen E. Flower, Christopher J. Caunt, Tony D. James, Robert J. Williams, Nicholas R. Waterfield, Jean M. H. Van Den Elsen

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22 Citations (SciVal)

Abstract

Glycated proteins are important biomarkers for age-related disorders, however their analysis is challenging because of the complexity of the protein-carbohydrate adducts. Here we report a method that enables the detection and identification of individual glycated proteins in complex samples using fluorescent boronic acids in gel electrophoresis. Using this method we identified glycated proteins in human serum, insect hemolymph and mouse brain homogenates, confirming this technique as a powerful proteomics tool that can be used for the identification of potential disease biomarkers.

Original language	English
Article number	1437
Pages (from-to)	1-7
Number of pages	7
Journal	Scientific Reports
Volume	3
DOIs	https://doi.org/10.1038/srep01437
Publication status	Published - 27 Mar 2013

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10.1038/srep01437Licence: CC BY-NC-ND

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author = "{Pereira Morais}, {Marta P.} and Dominic Marshall and Flower, {Stephen E.} and Caunt, {Christopher J.} and James, {Tony D.} and Williams, {Robert J.} and Waterfield, {Nicholas R.} and {Van Den Elsen}, {Jean M. H.}",

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AU - Flower, Stephen E.

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AU - James, Tony D.

AU - Williams, Robert J.

AU - Waterfield, Nicholas R.

AU - Van Den Elsen, Jean M. H.

PY - 2013/3/27

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AB - Glycated proteins are important biomarkers for age-related disorders, however their analysis is challenging because of the complexity of the protein-carbohydrate adducts. Here we report a method that enables the detection and identification of individual glycated proteins in complex samples using fluorescent boronic acids in gel electrophoresis. Using this method we identified glycated proteins in human serum, insect hemolymph and mouse brain homogenates, confirming this technique as a powerful proteomics tool that can be used for the identification of potential disease biomarkers.

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Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis

Abstract

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