Analysis of intestinal microbiota in hybrid house mice reveals evolutionary divergence in a vertebrate hologenome

Jun Wang, Shirin Kalyan, Natalie Steck, Leslie M. Turner, Bettina Harr, Sven Künzel, Marie Vallier, Robert Häsler, Andre Franke, Hans-Heinrich Oberg, Saleh M. Ibrahim, Guntram A. Grassl, Dieter Kabelitz, John F. Baines

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Recent evidence suggests that natural selection operating on hosts to maintain their microbiome contributes to the emergence of new species, that is, the 'hologenomic basis of speciation'. Here we analyse the gut microbiota of two house mice subspecies, Mus musculus musculus and M. m. domesticus, across their Central European hybrid zone, in addition to hybrids generated in the lab. Hybrid mice display widespread transgressive phenotypes (that is, exceed or fall short of parental values) in a variety of measures of bacterial community structure, which reveals the importance of stabilizing selection operating on the intestinal microbiome within species. Further genetic and immunological analyses reveal genetic incompatibilities, aberrant immune gene expression and increased intestinal pathology associated with altered community structure among hybrids. These results provide unique insight into the consequences of evolutionary divergence in a vertebrate 'hologenome', which may be an unrecognized contributing factor to reproductive isolation in this taxonomic group.

Original languageEnglish
Article number6440
Pages (from-to)1-10
Number of pages10
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 4 Mar 2015

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vertebrates
Pathology
Gene expression
mice
Vertebrates
divergence
incompatibility
phenotype
hybrid structures
gene expression
pathology
isolation
Reproductive Isolation
Bacterial Structures
Genetic Selection
Microbiota
Phenotype
Gene Expression
Gastrointestinal Microbiome

Keywords

  • Animals
  • Base Sequence
  • Biological Evolution
  • Crosses, Genetic
  • DNA Primers
  • Flow Cytometry
  • Gastrointestinal Microbiome
  • Genetics, Population
  • Genome
  • Germany
  • Hybridization, Genetic
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Quantitative Trait Loci
  • Selection, Genetic
  • Sequence Analysis, DNA
  • Species Specificity
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Analysis of intestinal microbiota in hybrid house mice reveals evolutionary divergence in a vertebrate hologenome. / Wang, Jun; Kalyan, Shirin; Steck, Natalie; Turner, Leslie M.; Harr, Bettina; Künzel, Sven; Vallier, Marie; Häsler, Robert; Franke, Andre; Oberg, Hans-Heinrich; Ibrahim, Saleh M.; Grassl, Guntram A.; Kabelitz, Dieter; Baines, John F.

In: Nature Communications, Vol. 6, 6440, 04.03.2015, p. 1-10.

Research output: Contribution to journalArticle

Wang, J, Kalyan, S, Steck, N, Turner, LM, Harr, B, Künzel, S, Vallier, M, Häsler, R, Franke, A, Oberg, H-H, Ibrahim, SM, Grassl, GA, Kabelitz, D & Baines, JF 2015, 'Analysis of intestinal microbiota in hybrid house mice reveals evolutionary divergence in a vertebrate hologenome', Nature Communications, vol. 6, 6440, pp. 1-10. https://doi.org/10.1038/ncomms7440
Wang, Jun ; Kalyan, Shirin ; Steck, Natalie ; Turner, Leslie M. ; Harr, Bettina ; Künzel, Sven ; Vallier, Marie ; Häsler, Robert ; Franke, Andre ; Oberg, Hans-Heinrich ; Ibrahim, Saleh M. ; Grassl, Guntram A. ; Kabelitz, Dieter ; Baines, John F. / Analysis of intestinal microbiota in hybrid house mice reveals evolutionary divergence in a vertebrate hologenome. In: Nature Communications. 2015 ; Vol. 6. pp. 1-10.
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