An ultra-high-performance liquid chromatography tandem mass spectrometry method for oxidative stress biomarker analysis in wastewater

Research output: Contribution to journalArticle

Abstract

Reported herein is the development of an analytical method for the detection of four oxidative stress biomarkers in wastewater using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) and solid phase extraction (SPE). The following four biomarkers of oxidative stress and lipid peroxidation have been investigated: hydroxynonenal-mercapturic acid (HNE-MA), 8-iso-prostglandin F2beta (8-iso-PGF 2β), 8-nitroguanine (8-NO 2Gua) and 8-hydroxy-2-deoxyguanosine (8-OHdG). The method showed very good performance: accuracy (> 87%), precision (> 90%), method quantification limits (1.3-3.0 ng L -1) and biomarker stability in wastewater in the case of HNE-MA, 8-OHdG and 8-iso-PGF 2β. In contrast, 8-NO 2Gua was found to be less stable in wastewater, which affected its method performance: accuracy (> 63%), precision (> 91%) and method quantification limits (85.3 ng L -1). Application of the developed method resulted in, for the first time, HNE-MA being successfully observed and quantified within wastewater over a study period of a week (displayed average daily loads per capita of 48.9 ± 4.1 mg/1000/people/day). 8-iso-PGF 2β was detected with good intensity but could not be quantified due to co-elution with other isomers. 8-OHdG was detected, albeit at < MQL. This study demonstrates the potential for expanding on the possible endogenous biomarkers of health used in urban water fingerprinting to aid in measuring health in near-real time on a community-wide scale.

LanguageEnglish
Pages2261-2271
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number11
Early online date23 Feb 2019
DOIs
StatusPublished - 19 Apr 2019

Keywords

  • Acetylcysteine/analysis
  • Aldehydes/analysis
  • Biomarkers/analysis
  • Chromatography, High Pressure Liquid/methods
  • Deoxyguanosine/analogs & derivatives
  • Guanine/analogs & derivatives
  • Humans
  • Limit of Detection
  • Lipid Peroxidation
  • Oxidative Stress
  • Prostaglandins F/analysis
  • Tandem Mass Spectrometry/methods
  • Waste Water/analysis
  • Water Pollutants, Chemical/analysis

Cite this

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title = "An ultra-high-performance liquid chromatography tandem mass spectrometry method for oxidative stress biomarker analysis in wastewater",
abstract = "Reported herein is the development of an analytical method for the detection of four oxidative stress biomarkers in wastewater using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) and solid phase extraction (SPE). The following four biomarkers of oxidative stress and lipid peroxidation have been investigated: hydroxynonenal-mercapturic acid (HNE-MA), 8-iso-prostglandin F2beta (8-iso-PGF 2β), 8-nitroguanine (8-NO 2Gua) and 8-hydroxy-2-deoxyguanosine (8-OHdG). The method showed very good performance: accuracy (> 87{\%}), precision (> 90{\%}), method quantification limits (1.3-3.0 ng L -1) and biomarker stability in wastewater in the case of HNE-MA, 8-OHdG and 8-iso-PGF 2β. In contrast, 8-NO 2Gua was found to be less stable in wastewater, which affected its method performance: accuracy (> 63{\%}), precision (> 91{\%}) and method quantification limits (85.3 ng L -1). Application of the developed method resulted in, for the first time, HNE-MA being successfully observed and quantified within wastewater over a study period of a week (displayed average daily loads per capita of 48.9 ± 4.1 mg/1000/people/day). 8-iso-PGF 2β was detected with good intensity but could not be quantified due to co-elution with other isomers. 8-OHdG was detected, albeit at < MQL. This study demonstrates the potential for expanding on the possible endogenous biomarkers of health used in urban water fingerprinting to aid in measuring health in near-real time on a community-wide scale.",
keywords = "Acetylcysteine/analysis, Aldehydes/analysis, Biomarkers/analysis, Chromatography, High Pressure Liquid/methods, Deoxyguanosine/analogs & derivatives, Guanine/analogs & derivatives, Humans, Limit of Detection, Lipid Peroxidation, Oxidative Stress, Prostaglandins F/analysis, Tandem Mass Spectrometry/methods, Waste Water/analysis, Water Pollutants, Chemical/analysis",
author = "Natalie Sims and Jack Rice and Barbara Kasprzyk-Hordern",
year = "2019",
month = "4",
day = "19",
doi = "10.1007/s00216-019-01667-8",
language = "English",
volume = "411",
pages = "2261--2271",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "11",

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TY - JOUR

T1 - An ultra-high-performance liquid chromatography tandem mass spectrometry method for oxidative stress biomarker analysis in wastewater

AU - Sims, Natalie

AU - Rice, Jack

AU - Kasprzyk-Hordern, Barbara

PY - 2019/4/19

Y1 - 2019/4/19

N2 - Reported herein is the development of an analytical method for the detection of four oxidative stress biomarkers in wastewater using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) and solid phase extraction (SPE). The following four biomarkers of oxidative stress and lipid peroxidation have been investigated: hydroxynonenal-mercapturic acid (HNE-MA), 8-iso-prostglandin F2beta (8-iso-PGF 2β), 8-nitroguanine (8-NO 2Gua) and 8-hydroxy-2-deoxyguanosine (8-OHdG). The method showed very good performance: accuracy (> 87%), precision (> 90%), method quantification limits (1.3-3.0 ng L -1) and biomarker stability in wastewater in the case of HNE-MA, 8-OHdG and 8-iso-PGF 2β. In contrast, 8-NO 2Gua was found to be less stable in wastewater, which affected its method performance: accuracy (> 63%), precision (> 91%) and method quantification limits (85.3 ng L -1). Application of the developed method resulted in, for the first time, HNE-MA being successfully observed and quantified within wastewater over a study period of a week (displayed average daily loads per capita of 48.9 ± 4.1 mg/1000/people/day). 8-iso-PGF 2β was detected with good intensity but could not be quantified due to co-elution with other isomers. 8-OHdG was detected, albeit at < MQL. This study demonstrates the potential for expanding on the possible endogenous biomarkers of health used in urban water fingerprinting to aid in measuring health in near-real time on a community-wide scale.

AB - Reported herein is the development of an analytical method for the detection of four oxidative stress biomarkers in wastewater using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) and solid phase extraction (SPE). The following four biomarkers of oxidative stress and lipid peroxidation have been investigated: hydroxynonenal-mercapturic acid (HNE-MA), 8-iso-prostglandin F2beta (8-iso-PGF 2β), 8-nitroguanine (8-NO 2Gua) and 8-hydroxy-2-deoxyguanosine (8-OHdG). The method showed very good performance: accuracy (> 87%), precision (> 90%), method quantification limits (1.3-3.0 ng L -1) and biomarker stability in wastewater in the case of HNE-MA, 8-OHdG and 8-iso-PGF 2β. In contrast, 8-NO 2Gua was found to be less stable in wastewater, which affected its method performance: accuracy (> 63%), precision (> 91%) and method quantification limits (85.3 ng L -1). Application of the developed method resulted in, for the first time, HNE-MA being successfully observed and quantified within wastewater over a study period of a week (displayed average daily loads per capita of 48.9 ± 4.1 mg/1000/people/day). 8-iso-PGF 2β was detected with good intensity but could not be quantified due to co-elution with other isomers. 8-OHdG was detected, albeit at < MQL. This study demonstrates the potential for expanding on the possible endogenous biomarkers of health used in urban water fingerprinting to aid in measuring health in near-real time on a community-wide scale.

KW - Acetylcysteine/analysis

KW - Aldehydes/analysis

KW - Biomarkers/analysis

KW - Chromatography, High Pressure Liquid/methods

KW - Deoxyguanosine/analogs & derivatives

KW - Guanine/analogs & derivatives

KW - Humans

KW - Limit of Detection

KW - Lipid Peroxidation

KW - Oxidative Stress

KW - Prostaglandins F/analysis

KW - Tandem Mass Spectrometry/methods

KW - Waste Water/analysis

KW - Water Pollutants, Chemical/analysis

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U2 - 10.1007/s00216-019-01667-8

DO - 10.1007/s00216-019-01667-8

M3 - Article

VL - 411

SP - 2261

EP - 2271

JO - Analytical and Bioanalytical Chemistry

T2 - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 11

ER -