An investigation into the stability of commercial
 versus MG63-derived hepatocyte growth factor under flow cultivation conditions

Giulia Meneghello, Michael P. Storm, Julian B. Chaudhuri, Paul A. De Bank, Marianne J. Ellis

Research output: Contribution to journalArticle


The scale-up of tissue engineering cell culture must ensure that conditions are maintained while also being cost effective. Here we analyse the stability of hepatocyte growth factor (HGF) to investigate whether concentrations change under dynamic conditions, and compare commercial recombinant human HGF as an additive in ‘standard medium’, to HGF secreted by the osteosarcoma cell line MG63 as a ‘preconditioned medium’. After 3 h under flow conditions, HGF in the standard medium degraded to 40 % of its original concentration but HGF in the preconditioned medium remained at 100 %. The concentration of secreted HGF was 10 times greater than the working concentration of commercially-available HGF. Thus HGF within this medium has increased stability; MG63-derived HGF should therefore be investigated as a cost-effective alternative to current lyophilised powders for use in in vitro models. Furthermore, we recommend that those intending to use HGF (or other growth factors) should consider similar stability testing before embarking on experiments with media flow.
Original languageEnglish
Pages (from-to)725-731
Number of pages7
JournalBiotechnology Letters
Issue number3
Early online date22 Oct 2014
Publication statusPublished - Mar 2015



  • Bioreactor
  • Hepatocyte growth factor
  • Liver
  • Perfusion
  • Regenerative medicine
  • Tissue engineering

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